CRISPR

CRISPR1-pax3a

ID

ZDB-CRISPR-220203-1

Name

CRISPR1-pax3a

Previous Names

None

Target

pax3a (1)

Sequence

5' - GGTAGTTTTGGGGTGGCGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
umu5	pax3a

Expression

Gene expression in Wild Types + CRISPR1-pax3a

No data available

Phenotype

Phenotype resulting from CRISPR1-pax3a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-pax3a

Phenotype	Fish	Conditions	Figures
integument pigmentation decreased process quality, abnormal	pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
integument pigmentation decreased process quality, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 1 from Nord et al., 2021
whole organism pax7b expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 7 from Nord et al., 2021
primitive pectoral fin abductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 3 from Nord et al., 2021
primitive pectoral fin adductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 3 from Nord et al., 2021
whole organism pax7a expression increased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5	standard conditions	Fig. 7 from Nord et al., 2021
integument pigmentation decreased process quality, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature decreased functionality, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 2 from Nord et al., 2021
trunk curved ventral, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 3 from Nord et al., 2021
primitive pectoral fin adductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 3 from Nord et al., 2021
whole organism lethal (sensu genetics), abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin abductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature cell population proliferation increased occurrence, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 4 from Nord et al., 2021
integument pigmentation decreased process quality, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin adductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
posterior hypaxial muscle myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin abductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature decreased functionality, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 2 from Nord et al., 2021
primitive pectoral fin abductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 3 from Nord et al., 2021
primitive pectoral fin abductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 3 , Fig. 4 from Nord et al., 2021
primitive pectoral fin abductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5	standard conditions	Fig. 4 from Nord et al., 2021
primitive pectoral fin abductor met expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor lbx1a expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor met expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature decreased functionality, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 2 from Nord et al., 2021
trunk curved ventral, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
whole organism lethal (sensu genetics), abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor lbx1a expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin abductor myog expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor myog expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid myog expression decreased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
myotome EGFP expression increased amount, abnormal	pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4; i150Tg	perforation: somite 10	Fig. 8 from Nord et al., 2021
primitive pectoral fin abductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor met expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature decreased functionality, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 2 from Nord et al., 2021
primitive pectoral fin abductor lacks all parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 3 from Nord et al., 2021
primitive pectoral fin adductor has fewer parts of type skeletal muscle cell, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 3 from Nord et al., 2021
whole organism lethal (sensu genetics), abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
trunk curved ventral, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 1 from Nord et al., 2021
primitive pectoral fin abductor lbx1a expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
pectoral fin musculature paralysed, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 2 from Nord et al., 2021
primitive pectoral fin abductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
primitive pectoral fin adductor myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
posterior hypaxial muscle myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021
sternohyoid myog expression decreased amount, abnormal	pax3b^umu6/umu6; pax7a^umu3/umu3; pax3a^umu5/umu5; pax7b^umu4/umu4	standard conditions	Fig. 5 from Nord et al., 2021

Citations