CRISPR

CRISPR2-megf6b

ID

ZDB-CRISPR-200528-16

Name

CRISPR2-megf6b

Previous Names

None

Target

megf6b (1)

Sequence

5' - TCCTGTATCCGGATGACAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Targets exon 31. The PAM site was "AGG" at the 3' end. This CRISPR contains the SNP rs514123267.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
z48	megf6b

Expression

Gene expression in Wild Types + CRISPR2-megf6b

No data available

Phenotype

Phenotype resulting from CRISPR2-megf6b

No data available

Phenotype of all Fish created by or utilizing CRISPR2-megf6b

Phenotype	Fish	Conditions	Figures
trunk melanocyte increased amount, abnormal	megf6b^z48/z48	standard conditions	Fig. 4 from Teerlink et al., 2020
post-vent region melanocyte increased amount, abnormal	megf6b^z48/z48	standard conditions	Fig. 4 from Teerlink et al., 2020
female organism increased weight, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 5 from Teerlink et al., 2020
ventral fin fold morphology, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 4 from Teerlink et al., 2020
mandibular arch skeleton developmental process delayed, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 5 from Teerlink et al., 2020
mandibular arch skeleton hypoplastic, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 5 from Teerlink et al., 2020
cleithrum hypoplastic, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 5 from Teerlink et al., 2020
vertebra ossification delayed, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 5 from Teerlink et al., 2020
post-vent region melanocyte increased amount, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 4 from Teerlink et al., 2020
caudal fin development disrupted, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 4 from Teerlink et al., 2020
caudal fin actinotrichium cartilage development delayed, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 5 from Teerlink et al., 2020
male organism decreased weight, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 5 from Teerlink et al., 2020
ceratobranchial 5 bone hypoplastic, abnormal	megf6b^z48/z48 + CRISPR1-megf6a + CRISPR2-megf6a	standard conditions	Fig. 5 from Teerlink et al., 2020

Citations