CRISPR

CRISPR3-cav1

ID

ZDB-CRISPR-200124-16

Name

CRISPR3-cav1

Previous Names

None

Target

cav1 (1)

Sequence

5' - GGTGGGCATCCCACTCGCCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
cn100	cav1
cn101	cav1

Expression

Gene expression in Wild Types + CRISPR3-cav1

No data available

Phenotype

Phenotype resulting from CRISPR3-cav1

No data available

Phenotype of all Fish created by or utilizing CRISPR3-cav1

Phenotype	Fish	Conditions	Figures
endocardium ab1-cav1 labeling absent, abnormal	cav1^cn100/cn100	cryoablation: cardiac ventricle	Figure 2 from Grivas et al., 2020
epicardium ab1-cav1 labeling absent, abnormal	cav1^cn100/cn100	cryoablation: cardiac ventricle	Figure 2 from Grivas et al., 2020
whole organism cav1 expression decreased amount, abnormal	cav1^cn100/cn100	standard conditions	Figure 2 from Grivas et al., 2020
heart valve Ab3-cav1 labeling absent, abnormal	cav1^cn100/cn100	cryoablation: cardiac ventricle	Figure 2 from Grivas et al., 2020
whole organism cav2 expression increased amount, abnormal	cav1^cn100/cn100	standard conditions	Figure 2 from Grivas et al., 2020
heart endothelial cell ab1-cav1 labeling absent, abnormal	cav1^cn100/cn100	cryoablation: cardiac ventricle	Figure 2 from Grivas et al., 2020
heart caveola absent, abnormal	cav1^cn100/cn100	standard conditions	Figure 3 from Grivas et al., 2020
endocardium Ab3-cav1 labeling absent, abnormal	cav1^cn100/cn100	cryoablation: cardiac ventricle	Figure 2 from Grivas et al., 2020
heart contraction decreased rate, abnormal	cav1^cn100/cn100	standard conditions	Figure 6 from Grivas et al., 2020
heart decreased functionality, abnormal	cav1^cn100/cn100	standard conditions	Figure 6 from Grivas et al., 2020
heart decreased elasticity, abnormal	cav1^cn100/cn100	standard conditions	Figure 6 from Grivas et al., 2020
epicardium Ab3-cav1 labeling absent, abnormal	cav1^cn100/cn100	cryoablation: cardiac ventricle	Figure 2 from Grivas et al., 2020
bulbus arteriosus Ab3-cav1 labeling absent, abnormal	cav1^cn100/cn100	cryoablation: cardiac ventricle	Figure 2 from Grivas et al., 2020
heart endothelial cell Ab3-cav1 labeling absent, abnormal	cav1^cn100/cn100	cryoablation: cardiac ventricle	Figure 2 from Grivas et al., 2020
heart caveola absent, abnormal	cav1^cn101/cn101	standard conditions	Figure S4 from Grivas et al., 2020
whole organism cav2 expression increased amount, abnormal	cav1^cn101/cn101	standard conditions	Figure S3 from Grivas et al., 2020
cardiac muscle cell Ab1-cavin1 labeling decreased amount, abnormal	cav1^cn100/cn100; li1Tg	cryoablation: cardiac ventricle	Figure S2 from Grivas et al., 2020
bulbus arteriosus Ab1-cavin1 labeling decreased amount, abnormal	cav1^cn100/cn100; li1Tg	cryoablation: cardiac ventricle	Figure S2 from Grivas et al., 2020
heart valve Ab1-cavin1 labeling absent, abnormal	cav1^cn100/cn100; li1Tg	cryoablation: cardiac ventricle	Figure S2 from Grivas et al., 2020

Citations