CRISPR

CRISPR11-shank3a

ID

ZDB-CRISPR-190807-1

Name

CRISPR11-shank3a

Previous Names

None

Target

shank3a (1)

Sequence

5' - GGAATCCTGCAGGATGATCATGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
umi101	shank3a

Expression

Gene expression in Wild Types + CRISPR11-shank3a

No data available

Phenotype

Phenotype resulting from CRISPR11-shank3a

No data available

Phenotype of all Fish created by or utilizing CRISPR11-shank3a

Phenotype	Fish	Conditions	Figures
intestinal motility disrupted, abnormal	AB + CRISPR11-shank3a + CRISPR2-shank3b	standard conditions	Fig. 3 from James et al., 2019
intestine peristalsis decreased frequency, abnormal	AB + CRISPR11-shank3a + CRISPR2-shank3b	standard conditions	Fig. 3 from James et al., 2019
intestinal motility decreased rate, abnormal	shank3a^umi101/+; shank3b^umi102/+ (AB)	standard conditions	Fig. 4 from James et al., 2019
intestine peristalsis decreased frequency, abnormal	shank3a^umi101/+; shank3b^umi102/+ (AB)	standard conditions	Fig. 3 from James et al., 2019
intestine has fewer parts of type enteroendocrine cell, abnormal	shank3a^umi101/+; shank3b^umi102/+ (AB)	standard conditions	Fig. 7 from James et al., 2019
intestinal bulb intestine smooth muscle contraction disrupted, abnormal	shank3a^umi101/+; shank3b^umi102/+ (AB)	standard conditions	Fig. 4 from James et al., 2019
intestinal motility disrupted, abnormal	shank3a^umi101/+; shank3b^umi102/+ (AB)	standard conditions	Fig. 3 from James et al., 2019
intestine has extra parts of type goblet cell, abnormal	shank3a^umi101/+; shank3b^umi102/+ (AB)	standard conditions	Fig. S2 from James et al., 2019
intestine peristalsis decreased frequency, abnormal	shank3a^{umi101/umi101}; shank3b^{umi102/umi102} (AB)	standard conditions	Fig. 3 from James et al., 2019
intestine has fewer parts of type enteroendocrine cell, abnormal	shank3a^{umi101/umi101}; shank3b^{umi102/umi102} (AB)	standard conditions	Fig. 7 from James et al., 2019
intestine has extra parts of type goblet cell, abnormal	shank3a^{umi101/umi101}; shank3b^{umi102/umi102} (AB)	standard conditions	Fig. S2 from James et al., 2019
intestine lumen decreased diameter, abnormal	shank3a^{umi101/umi101}; shank3b^{umi102/umi102} (AB)	standard conditions	Fig. S2 from James et al., 2019
intestinal motility disrupted, abnormal	shank3a^{umi101/umi101}; shank3b^{umi102/umi102} (AB)	standard conditions	Fig. 3 from James et al., 2019
intestinal villus swollen, abnormal	shank3a^{umi101/umi101}; shank3b^{umi102/umi102} (AB)	standard conditions	Fig. S2 from James et al., 2019

Citations