Events followed during Axolotl’s Limb Regeneration. (1) Amputation plane where limbs can be amputated to witness the events leading to regeneration. (2) Formation of wound epidermis followed by initial immune response for clearing the pathogens and unwanted mass. (3) Nerve formation is essential for blastema formation and growth. (4) Blastema is the mass of de-differentiated cells necessary for regeneration. (5) Blastema cells proliferate and differentiate into cells of choice to develop into new limbs, looking like the original ones. (6) Patterning of limbs starts that gives rise to new limbs just like the original ones.

Bone Remodeling: Hormonal regulation of signaling pathways and molecules involved. Bone resorption: Receptor Activator of Nuclear factor Kappa beta Ligand (RANKL) calcitonin and TGF-β are involved in the activation of osteoclasts, promoting bone resorption. Reversal: Resorption is followed by a reversal process where TGF-β, IGF1, 2, BMPs, and FGFs play a central role and activate osteocytes. Bone formation: After activation, osteocytes lead to bone formation involving molecules including Wnt, IGF, BMP, PTH, RUNX2, TGF-β, and FGF 23.