Fig. 1

Schematic illustration of toxin and drug treatment for the neuro-restoration protocol. One day post fertilization (dpf), dechorionated zebrafish were treated for 48 hours with 500 μM 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). MPTP was replaced with candidate drugs and treatment was continued for another 48 hours. After treatment, in vivo imaging and behavioral analyses were performed to reveal the neuro-restorative effects of the drugs following MPTP exposure.

Fig. 2

Zebrafish preparation for quantification of dopaminergic neurons. A : Immunostaining for tyrosine hydroxylase (TH) on horizontal cryosection of Tg(dat:EGFP) larvae at 3 days post-fertilization (dpf) following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment for 48 hours from 1 dpf with each MPTP concentration. B : In vivo imaging technique by fixing the whole larva ventrally. C : In vivo imaging technique by fixing the heads of larvae. Representative morphology of dopaminergic neurons in Tg(dat:EGFP) by in vivo imaging with each MPTP concentration. Ventral view, head at the left, tectum at the right.

Fig. 3

The maximum concentration of the drugs that did not induce any morphological changes. A : Levodapa 1 mmol. B : Nifedipine 10 μmol. C : Nimodipine 3.5 μmol. D : Diethylstilbestrol 0.3 μmol. E : Calcitriol 0.25 μmol. F : Luteolin 100 μmol.

Fig. 4

Locomotor behavior analysis for 20 minutes under light conditions. *p<0.05 vs. the MPTP followed by DMSO-treated group. E3 : embryo medium (5 mmol NaCl, 0.17 mmol KCl, 0.33 mmol CaCl2, 0.33 mmol MgSO4), DMSO : dimethylsulfoxide, DES : diethylstilbestrol, L-DOPA : levodopa, NIFE : nifedipine.

Fig. 5

Representative morphology of dopaminergic neurons in Tg(dat:EGFP) in vivo imaging with each drug. Scale bars (gray line), 100 μm. Ventral view, head at the left, tectum at the right. DMSO : dimethylsulfoxide, DES : diethylstilbestrol.

Fig. 6

Quantitative analysis of dopaminergic neurons in Tg(dat:EGFP) zebrafish ventral diencephalon. Data are expressed as a percentage of the control group. Each bar represents mean±standard deviation. *p<0.05 vs. the MPTP followed by DMSO-treated group. DMSO : dimethylsulfoxide, DES : diethylstilbestrol.

Fig. Abs

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Korean Neurosurg Soc
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.