Fig. 1

Zebrafish survival rate 24, 48 and 72 h after Klebsiella pneumoniae infection with different virulence. Error bars are presented as the SEM. Values are presented as the mean ± SEM of three replicates ( ****P < 0.0001)

PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult
Fig. 2

Analysis of intestinal colonization of zebrafish 48 h after Klebsiella pneumoniae infection with different virulence. A Representative plate in each groups. B CFU statistics in the intestines of zebrafish infected by Klebsiella pneumoniae with different virulence. Error bars are presented as the SEM. Values are presented as the mean ± SEM of three replicates (**P < 0.01, ***P < 0.001,)
Fig. 3

Changes in the structure of the gut 48 h after Klebsiella pneumoniae infected zebrafish with different virulence. The intestinal tract after infection was subjected to hematoxylin and eosin staining. A Control, (B) KP1052. C KP1196, and (D) KP1195. The red arrow indicates was goblet cells and the white arrows was epithelial cells. scale bars: 10μm
Fig. 4

Changes in the content of inflammatory factors in the intestine of zebrafish after infection with Klebsiella pneumoniae of different virulence; 48 h after K. pneumoniae infection, the contents of (A) Il-1ɑ, (B) Il-1β, and (C) TNF-ɑ were determined by ELISA. Error bars are presented as the SEM. Values are presented as the mean ± SEM of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001)
Fig. 5

After infecting zebrafish with Klebsiella pneumoniae of different virulence, 16S rRNA gene sequencing was used to detect the composition of microorganisms in the intestine. A The Venn chart shows the number of OTUs shared and unique to different experimental groups. B Profiles of zebrafish gut microbes at the phylum level in different experimental groups. C Profiles of zebrafish gut microbes at the top phylum level in different experimental groups. D The composition of Top 10 species of zebrafish gut microbes in different experimental groups
Fig. 6

The Shannon index of gut microbial diversity after different strains of Klebsiella pneumoniae infected zebrafish
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Infect Dis
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