PUBLICATION
Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity
- Authors
- Wang, X., Li, T., Zhou, L., Tu, F., Rui, X., Xu, Z., Liu, J., Cao, F.
- ID
- ZDB-PUB-231222-12
- Date
- 2023
- Source
- BMC infectious diseases 23: 899899 (Journal)
- Registered Authors
- Keywords
- Inflammation, Intestine, Klebsiella pneumonia, Microbial diversity, Zebrafish
- MeSH Terms
-
- Humans
- Klebsiella pneumoniae*
- Microbial Sensitivity Tests
- Virulence
- Klebsiella Infections*/microbiology
- Zebrafish
- Animals
- Anti-Bacterial Agents
- Adult
- Inflammation
- Intestines/microbiology
- PubMed
- 38129788 Full text @ BMC Infect Dis
Citation
Wang, X., Li, T., Zhou, L., Tu, F., Rui, X., Xu, Z., Liu, J., Cao, F. (2023) Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity. BMC infectious diseases. 23:899899.
Abstract
Background Klebsiella pneumoniae is a pathogen that often infects patients in clinical practice. Due to its high virulent and drug resistance, infected patients are difficult to treat. In clinical practice, Klebsiella pneumoniae can infect patients' intestines, intestines, blood, etc., causing pathological changes. However, there is relatively little information on the impact of Klebsiella pneumoniae on intestinal inflammation and microbial populations. Zebrafish is an excellent biomedical model that has been successfully applied to the virulence assessment of Klebsiella pneumoniae.
Methods In this study, three clinically isolated representative strains of Klebsiella pneumoniae (high virulence non-resistant, high virulence resistant, and low virulence resistant) were used to infect zebrafish, and their effects on intestinal colonization, inflammation, pathology, and microbial diversity were tested.
Results Enzyme-linked immunoassay (ELISA) showed that Klebsiella pneumoniae significantly increased levels of the cytokines interleukin-1α (Il-1α), interleukin-1β (Il-1β), and tumor necrosis factor-α (Tnf-α), which increased inflammatory symptoms. Hematoxylin eosin staining(H&S) showed that Klebsiella pneumoniae treatment caused intestinal lesions in zebrafish, in which KP1053 exposure significantly decreased the number of goblet cells, KP1195 caused epithelial dissolution and exfoliation. In addition, Klebsiella pneumoniae disturbed the composition of intestinal microbiota, and the Shannon index increased, which increased the number of harmful bacteria.
Conclusions Klebsiella pneumoniae infection can lead to intestinal colonization, inflammation, pathological changes, and changes in microbial biodiversity. This study provides a reference for the intestinal pathology of clinical Klebsiella pneumoniae infection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping