Fig. 1

(a) Fluorescence spectra of 10 ?M P-1 in different ratios of W (water):G (glycerol). (b) Linearity of log (I670) vs log (viscosity). ?ex = 590 nm.

Fig. 2

(a) Absorption and (b) fluorescence spectra of 1 (10 ?M) and P-1 (10 ?M) in the absence and presence of ONOO?. (c) Fluorescence intensity of P-1 reacting with ONOO? for different time periods. (d) Time-dependent fluorescence intensity of P-1 to ONOO?. (e) Fluorescence response of P-1 addition with different concentrations of ONOO? (30?870 nM). (f) Fluorescence intensities of P-1 as a function of ONOO? concentrations. Slit width: dex = dem = 5 nm.

Fig. 3

Colocalization of 1.5 ?M MitoTracker Green (a), 3 ?M P-1 (b, f), and LysoTracker Green DND-26 (e) in HeLa cells. (c, g) Merged images. (d) Intensity scattering plot of P-1 (Ex = 568 nm, Em = 650?720 nm) with MitoTracker Green (Ex = 488 nm, Em = 490?530 nm). (h) Intensity scattering plot of P-1 with LysoTracker Green DND-26 intensities.

Fig. 4

(a) Cell imaging of P-1 (3 ?M) induced by nystatin (5 ?g/mL) in HeLa and SMMC-7721 cells. (b) Mean fluorescence intensities of P-1 in living cells. ?ex = 568 nm, ?em = 650?720 nm.

Fig. 5

(a) Cell imaging of P-1 (3 ?M) induced by LPS for 1, 2, and 3 h, separately. (b) Time dependence of mean fluorescence intensity. (c) Real-time cell imaging of P-1 (3 ?M) under starvation conditions. (d) Time dependence of mean fluorescence intensity changes of P-1. P-1: ?ex = 568 nm, ?em = 650?720 nm. Scale bar: 20 ?m.

Fig. 6

Confocal fluorescence imaging of ONOO? in RAW 264.7 cells. (a) RAW 264.7 cells were incubated with P-1 (3 ?M). (b) RAW 264.7 cells were loaded with SIN-1 (100 ?M) for 30 min and then with P-1 for 1 h. (c) Cells were incubated with LPS (100 ?M) for 4 h, IFN-? (100 ng/mL) and PMA (10 nM) for 30 min, and then with P-1 for 1 h. (d) Cells were incubated with ebselen (100 ?M) for 2 h, LPS for 4 h, IFN-? and PMA for 30 min, and then with P-1 for 1 h. (e?h) Merged images. (i) Mean fluorescence intensities of panels (e?h). ?ex = 405 nm, ?em = 550?620 nm.

Fig. 7

(a?c) Fluorescence images of only P-1 (3 ?M) in zebrafish. (d?f) Fluorescence images of P-1 (3 ?M) with nystatin (5 ?g/mL) in the zebrafish. (g) Time-dependent mean fluorescence intensity changes of P-1. ?ex = 568 nm, ?em = 650?720 nm.

Fig. 8

In vivo fluorescence imaging of exogenous ONOO? in zebrafish. (a?c) Fluorescence images of the zebrafish incubated with only P-1 (3 ?M). (d?f) Fluorescence images of P-1 (3 ?M) with SIN-1 (500 ?M) in zebrafish. (g) Time-dependent mean fluorescence intensity changes of P-1. ?ex = 568 nm, ?em = 650?720 nm.

Acknowledgments
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