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Fig. 6 Confocal fluorescence imaging of ONOO? in RAW 264.7 cells. (a) RAW 264.7 cells were incubated with P-1 (3 ?M). (b) RAW 264.7 cells were loaded with SIN-1 (100 ?M) for 30 min and then with P-1 for 1 h. (c) Cells were incubated with LPS (100 ?M) for 4 h, IFN-? (100 ng/mL) and PMA (10 nM) for 30 min, and then with P-1 for 1 h. (d) Cells were incubated with ebselen (100 ?M) for 2 h, LPS for 4 h, IFN-? and PMA for 30 min, and then with P-1 for 1 h. (e?h) Merged images. (i) Mean fluorescence intensities of panels (e?h). ?ex = 405 nm, ?em = 550?620 nm.
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