Figure 1

Response surface plots (A–C) and contour plots (a–c) showing the effects of processing temperature, processing time, amount of HCl and their mutual effects on the extraction yield of PFSV (R1).
Figure 2

Elution curve of crude PFSV on DEAE-52 cellulose and sephacryl S-400HR chromatography column (A); FT-IR spectrum of PFSV-2 (B); and chromatograms of the molar mass distribution of PFSV-2 (C).
Figure 3

The 1H-NMR (A), 13C (B) NMR, 1H-1H COSY (C), NOESY (D), HSQC (E), and HMBC (F) spectra of PFSV-2. The predicted structure of PFSV-2 (G).
Figure 4

Total cholesterol [TC, (A)] and triglyceride [TG, (B)] levels of larval zebrafish in each group (n = 3); mRNA expression of pparg, fasn, and mtp on zebrafish larvae (C); mRNA expression of pparab and acaca on zebrafish larvae (D), mRNA expression of HMGCRa and HMGCRb on zebrafish larvae (E), and molecular mechanisms of lipid metabolism modulation by PFSV-2 (F). Bar indicates means ± SD. Bars without the same superscripts (a?e) denote significant difference (p < 0.05).
Acknowledgments
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