PUBLICATION
Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method
- Authors
- Zhang, Z.F., Song, T.T., Chen, J.F., Lv, G.Y.
- ID
- ZDB-PUB-230131-13
- Date
- 2023
- Source
- Frontiers in nutrition 9: 10955561095556 (Journal)
- Registered Authors
- Keywords
- Sanghuangporus vaninii, acid-ethanol pretreatment, hypolipidemic polysaccharide, structure illustration, zebrafish model
- MeSH Terms
- none
- PubMed
- 36712537 Full text @ Front Nutr
Citation
Zhang, Z.F., Song, T.T., Chen, J.F., Lv, G.Y. (2023) Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method. Frontiers in nutrition. 9:10955561095556.
Abstract
In this study, an effective method was developed to extract the polysaccharide from Sanghuangporus vaninii (PFSV) by destroying the cell wall. Box-Behnken design was employed to determine the optimal processing conditions as follows: processing temperature (80°C), processing time (0.81 h) and amount of HCl (1.5 ml). Under these conditions, the yield of PFSV reached 5.94 ± 0.16%. The purified polysaccharide (PFSV-2) was found to be a hetero-polysaccharide with an average molecular weight of 20.377 kDa. The backbone of PFSV-2 was composed of an →6)-α-Galp-(1→ and →2,6)-β-Manp-(1→ and →2)-α-Fucp-(1→ and was branched of t-α-Manp-(1→ at position 2 of residue B. PFSV-2 showed hypolipidemic activity by decreasing lipid accumulation and the levels of total cholesterol and triglycerides in zebrafish larvae. Furthermore, PFSV-2 downregulated the pparg, fasn, and HMGCRb genes and upregulated the pparab and acaca genes. These findings suggested PFSV-2 may be a promising candidate in lipid regulation therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping