- Title
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Comparative Studies of Renin-Null Zebrafish and Mice Provide New Functional Insights
- Authors
- Hoffmann, S., Mullins, L., Rider, S., Brown, C., Buckley, C.B., Assmus, A., Li, Z., Sierra Beltran, M., Henderson, N., Del Pozo, J., De Goes Martini, A., Sequeira-Lopez, M.L.S., Gomez, R.A., Mullins, J.
- Source
- Full text @ Hypertension
AMA analysis.
A) Quantification of the LifeActRFP fluorescent signal at the AMA in Tg(ren:LifeAct-RFP;acta2:EGFP) 5dpf larvae on a ren +/+ or ren -/- background (n=5 per group); B) SPIM microscopy of pronephric AMA showing LifeActRFP (Ren-expressing cells) and EGFP (Acta2-expressing cells) fluorescence and C) representative greyscale image for mean area analysis. Laser ablation of renin-expressing cells in the AMA of Tg(ren:mem-KillerRed) 3dpf larvae showing kdrl:GFP signals at D) t = 0min and E) t = 60mins of the ablation protocol. The dorsal aorta (DA) can be seen with the anterior mesenteric artery (AMA) budding off it. Images represent single axial planes. Scale bars represent 30μm; AMA diameter was measured at the same two locations for each fish (dotted lines); F) An Estimation plot and mean of differences are shown; (Paired t-test*: p = 0.0147) |
Spinning disc microscopy showing the expression of fluorescent reporters ren:LifeAct-RFP and acta2:EGFP in mesonephric kidney squashes from A) ren +/+, B) ren -/- or the obligate heterozygous fish C) ren -/Δ9, and D) ren -/KI. Panels show separate red, green and merged channels. Scale bar = 50μm. |
Merged expression matrices from A) wild-type mouse and zebrafish JG cells and B) renin knockout mouse and zebrafish renin lineage cells following CCA analysis (resolution 0.5 and 0.6 respectively). Associated tables give contributing cell numbers from respective matrices; C) and D) genes differentially expressed between respective mouse and zebrafish libraries, irrespective of genotype, are shown in dot plots. |