PUBLICATION
Comparative Studies of Renin-Null Zebrafish and Mice Provide New Functional Insights
- Authors
- Hoffmann, S., Mullins, L., Rider, S., Brown, C., Buckley, C.B., Assmus, A., Li, Z., Sierra Beltran, M., Henderson, N., Del Pozo, J., De Goes Martini, A., Sequeira-Lopez, M.L.S., Gomez, R.A., Mullins, J.
- ID
- ZDB-PUB-220111-8
- Date
- 2022
- Source
- Hypertension (Dallas, Tex. : 1979) 79(3): e56-e66 (Journal)
- Registered Authors
- Mullins, John, Rider, Sebastien
- Keywords
- actin, glycosylation, pericytes, renin, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Blood Pressure/genetics*
- Clustered Regularly Interspaced Short Palindromic Repeats
- Kidney/metabolism*
- Mice
- Mice, Knockout
- Renin/genetics
- Renin/metabolism*
- Renin-Angiotensin System/physiology*
- Transcriptome*
- Zebrafish
- PubMed
- 35000430 Full text @ Hypertension
Citation
Hoffmann, S., Mullins, L., Rider, S., Brown, C., Buckley, C.B., Assmus, A., Li, Z., Sierra Beltran, M., Henderson, N., Del Pozo, J., De Goes Martini, A., Sequeira-Lopez, M.L.S., Gomez, R.A., Mullins, J. (2022) Comparative Studies of Renin-Null Zebrafish and Mice Provide New Functional Insights. Hypertension (Dallas, Tex. : 1979). 79(3):e56-e66.
Abstract
Background The renin-angiotensin system is highly conserved across vertebrates, including zebrafish, which possess orthologous genes coding for renin-angiotensin system proteins, and specialized mural cells of the kidney arterioles, capable of synthesising and secreting renin.
Methods We generated zebrafish with CRISPR-Cas9-targeted knockout of renin (ren-/-) to investigate renin function in a low blood pressure environment. We used single-cell (10×) RNA sequencing analysis to compare the transcriptome profiles of renin lineage cells from mesonephric kidneys of ren-/- with ren+/+ zebrafish and with the metanephric kidneys of Ren1c-/- and Ren1c+/+ mice.
Results The ren-/- larvae exhibited delays in larval growth, glomerular fusion and appearance of a swim bladder, but were viable and withstood low salinity during early larval stages. Optogenetic ablation of renin-expressing cells, located at the anterior mesenteric artery of 3-day-old larvae, caused a loss of tone, due to diminished contractility. The ren-/- mesonephric kidney exhibited vacuolated cells in the proximal tubule, which were also observed in Ren1c-/- mouse kidney. Fluorescent reporters for renin and smooth muscle actin (tg(ren:LifeAct-RFP; acta2:EGFP)), revealed a dramatic recruitment of renin lineage cells along the renal vasculature of adult ren-/- fish, suggesting a continued requirement for renin, in the absence of detectable angiotensin metabolites, as seen in the Ren1YFP Ren1c-/- mouse. Both phenotypes were rescued by alleles lacking the potential for glycosylation at exon 2, suggesting that glycosylation is not essential for normal physiological function.
Conclusions Phenotypic similarities and transcriptional variations between mouse and zebrafish renin knockouts suggests evolution of renin cell function with terrestrial survival.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping