FIGURE SUMMARY
Title

Use of melatonin as an inhibitor of apoptotic process for cryopreservation of zebrafish (Danio rerio) embryos

Authors
Castro, P.L., Ferraz, A.L.J., Patil, J.G., Ribeiro, R.P.
Source
Full text @ Braz. J. Biol.
Fig. 1

Representative scanning electron micrographs of vitrified and non-vitrified zebrafish (Danio rerio) embryos. (A) Control group; (B-D) Vitrified with 0, 1 µM and 1 mM melatonin respectively. Arrows, arrow head and asterisk point to invaginations and perforations, rupture of the vitelline membrane and wrinkling of the epidermis respectively.

Fig. 2

Bax, bcl-2 and caspase-3 mRNA expression levels in zebrafish (Danio rerio) embryos for the control group and vitrified treatments with 0, 1 µM and 1 mM melatonin respectively obtained by the 2-ΔΔCT method. Boxes with same letter are not significantly different from one another (P > 0.05).

Fig. 3

Percentage area of zebrafish (Danio rerio) embryos affected by reactive oxygen species (ROS) formation and DNA fragmentation after vitrification for the control group and vitrified treatments with 0, 1 µM and 1 mM melatonin respectively. Bars with different letter within the same assay were significantly different from one another (P < 0.05).

Fig. 4

Representative images of zebrafish (Danio rerio) embryos showing reactive oxygen species (ROS) formation (A-D) and DNA fragmentation (E-H) following vitrification for the control group and vitrified treatments with 0, 1 µM and 1 mM melatonin respectively. Arrows and arrow heads point to tissues affected in the body axis and those in close proximity to the yolk respectively.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Braz. J. Biol.
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