Fig. 2

Fig. 2. Fluorescence microscopy of tissues from adult zebrafish exposed to different concentrations of polystyrene microplastics for 21 days. Polystyrene microplastics in green fluorescence are indicated with red arrows and borders. Scale bar: 1 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 3

Fig. 3. Steroidogenic mRNA expression in ovary (A&B) and testis (C&D) tissues of adult zebrafish after exposure to different concentrations of polystyrene microplastics for 21 days; steroidogenesis-related genetic pathway in adult zebrafish gonads (E). Expression levels were calculated using the 2-??CT method. Error bars represent the mean ± standard error. Asterisks represent significant difference from the control (One-way ANOVA followed by a Dunnett's post-hoc test, *p < 0.05, **p < 0.01). n = 4 female/male fish.

Fig. 4

Fig. 4. The 17 ?-estradiol (A) and testosterone (B) concentrations and ratio (C) in adult zebrafish after exposure to different concentrations of polystyrene microplastics for 21 days. Error bars represent the mean ± standard error. n = 4 female/male fish.

Fig. 5

Fig. 5. Cumulative number of eggs produced per adult female zebrafish and fertilization rate after (A & D) 10 days of pre-exposure, followed by (B & E) 21 days of exposure to polystyrene microplastics at different concentrations. (C) Average number of eggs produced per adult female zebrafish and (F) average fertilization rate upon parental exposure to polystyrene microplastics at different concentrations for 21 days. Error bars represent the mean ± standard error. n = 4 tanks.

Fig. 6

Fig. 6. Hatching rate (A), body length (B), malformation rate (C) and mortality rate (D) of embryonic zebrafish with parental exposure to different concentrations of polystyrene microplastics for 21 days. Hatching rates were calculated and measured at 72 hpf; body length, malformation, and mortality rate were calculated and measured at 96 hpf. Error bars represent the mean ± standard error. n = 4 tanks.

Acknowledgments
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