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Fig. 3 Fig. 3. Steroidogenic mRNA expression in ovary (A&B) and testis (C&D) tissues of adult zebrafish after exposure to different concentrations of polystyrene microplastics for 21 days; steroidogenesis-related genetic pathway in adult zebrafish gonads (E). Expression levels were calculated using the 2-??CT method. Error bars represent the mean ± standard error. Asterisks represent significant difference from the control (One-way ANOVA followed by a Dunnett's post-hoc test, *p < 0.05, **p < 0.01). n = 4 female/male fish.
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