Figure 1

Ultraviolet–visible spectra (A) and Flourier transformation infrared spectra (B) of theabrownins (TBs) and fractionated theabrownin samples with different molecular weights. TBs-LT3k, TBs-3-10k, TBs-10-30k, and TBs-GT30k represented the theabrownin samples with molecular weights: <3 kDa, 3–10 kDa, 10–30 kDa, and >30 kDa, respectively.
Figure 2

Hypolipidemic effect of theabrownins (TBs) and fractionated theabrownin samples with different molecular weights in high-fat-induced obesity zebrafish. (A) Experimental outline of zebrafish model. (B) Zebrafish stained with oil red O and visualized under a microscope magnified 30 times. (C–E) Relative lipid accumulation in zebrafish treated with different concentrations of theabrownins. Data were expressed as mean ± SEM (n = 6). *p-value < 0.05 and #p-value < 0.001 compared to high-fat diet (HFD) group. HFD, high-fat diet-induced obesity zebrafish. DMSO, vehicle control at 0.1% (v/v). Simvastatin, positive control at 0.06 μM. TBs-LT3k, TBs-3-10k, and TBs-10-30k represented the fractionated theabrownin samples with molecular weights: <3 kDa, 3–10 kDa, and 10–30 kDa, respectively. Scale bar, 500 μm.
Figure 3

Thermogravimetry/Differential thermogravimetry analysis (A) and differential scanning calorimetry (B) analysis results of TBs-10-30k.
Figure 4

X-ray diffraction pattern of TBs-10-30k.
Figure 5

Morphological characteristics of TBs-10-30k. (A) Images magnified by scanning electron microscopy at 500 and 1000 times, respectively. (B) Planar images and corresponding three-dimensional images of TBs-10-30k obtained by atomic force microscopy. The scanning range is 5 × 5 μm and 1 × 1 μm, respectively.
Acknowledgments
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