Biodistribution after PSNP exposure in zebrafish larvae from 72 to 120?hpf. a Representative pictures of PSNP accumulation in the intestine (lateral view, solid arrow), exocrine pancreas (lateral view, empty arrow), and b gallbladder (ventral and lateral view, asterisk) of wild-type zebrafish larvae. The experiment was conducted on three separate occasions with ten biologically independent replicates each

Effects of PSNPs on glucose metabolism in larval zebrafish. a Dose-dependent response showing glucose concentrations in zebrafish embryos wild-type (AB/TL and gr+/+), glucocorticoid receptor mutant (gr?/?), and AB/TL supplemented with the glucocorticoid receptor antagonist mifepristone (1?µM; n?=?4?5, pool of ten zebrafish per sample). b Representative image of insulin staining of the pancreas in control and 20?mg?L^?1 PSNP-exposed embryos (n?=?7, biologically independent replicates). c Dose-response showing pck1 promoter dynamics in Tg(pck1:Luc2)zebrafish larvae at 120?hpf after exposure to 0.2?mg?L^?1 (low), 2?mg?L^?1 (mid), and 20?mg?L^?1(high) PSNPs from 72 to 120?hpf (n?=?5, pool of three zebrafish per sample). Values are presented as mean?±?SD. Asterisks indicate significant differences to controls (*p?<?0.05, **p?<?0.01, and ***p?<?0.001)