FIGURE SUMMARY
Title

Functional characterization of Cynoglossus semilaevis R-spondin2 and its role in muscle development during embryogenesis

Authors
Wang, B., Yang, F., Li, R., Li, X., Wu, X., Sun, Z., Zhai, J., He, Y., Qi, J.
Source
Full text @ Genes Genet. Sys.
Fig. 5

Whole-mount in situ hybridization (WISH) of the zebrafish muscle-specific gene MyoD after injection of embryos with CsRspo2 mRNA. (A) Positive signals from antisense probe hybridization are purple or blue. (a, a1) Control group with normally developed embryos at the 11?12-somite stages, (b) embryo injected with CsRspo2 mRNA, and (c) sense probe ISH. (B) Abnormal embryos showing slow development after injection. MyoD mRNA-positive signals are indicated by black arrows. Scale bars = 50 ?m.

Fig. 6

Immunostaining of whole-mount zebrafish embryos by anti-MyHC for slow muscles (F59). Embryos were injected with RNA-free water or 200 pg CsRspo2 mRNA. (A) Slow fibrils in control embryos. (B, C, D) Organization of filaments in slow muscles of CsRspo2 mRNA-injected zebrafish embryos was severely defective (arrows). Scale bars = 50 ?m.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Genes Genet. Sys.
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