PUBLICATION
Functional characterization of Cynoglossus semilaevis R-spondin2 and its role in muscle development during embryogenesis
- Authors
- Wang, B., Yang, F., Li, R., Li, X., Wu, X., Sun, Z., Zhai, J., He, Y., Qi, J.
- ID
- ZDB-PUB-181020-6
- Date
- 2018
- Source
- Genes & genetic systems 93(5): 181-190 (Journal)
- Registered Authors
- He, Yan, Qi, Jie, Wang, Bo
- Keywords
- Cynoglossus semilaevis, R-spondin2, Wnt signaling pathway, muscle development
- MeSH Terms
-
- Cloning, Molecular
- Xenopus Proteins/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology*
- Muscle Development/genetics*
- Muscle Development/physiology
- Male
- Amino Acid Sequence
- Animals
- Intercellular Signaling Peptides and Proteins/genetics*
- Intercellular Signaling Peptides and Proteins/physiology*
- Embryonic Development
- Phylogeny
- RNA, Messenger/genetics
- Zebrafish
- Female
- Flatfishes/embryology
- Flatfishes/genetics
- Flatfishes/physiology
- PubMed
- 30333384 Full text @ Genes Genet. Sys.
Citation
Wang, B., Yang, F., Li, R., Li, X., Wu, X., Sun, Z., Zhai, J., He, Y., Qi, J. (2018) Functional characterization of Cynoglossus semilaevis R-spondin2 and its role in muscle development during embryogenesis. Genes & genetic systems. 93(5):181-190.
Abstract
R-spondin2 (Rspo2) is a member of the R-spondin family, which plays important roles in cell proliferation, cell fate determination and organogenesis. Rspo2 exhibits important functions during embryonic development and muscle maintenance in adult human, mouse and Xenopus. In the present study, the tongue sole Cynoglossus semilaevis Rspo2 (CsRspo2) gene was isolated and characterized, and its role in muscle development during embryogenesis was studied. Our results showed that CsRspo2 expression was abundant during gastrulation and significantly high during somite formation, but then decreased markedly after hatching. CsRspo2 expression was high in brain and gill, moderate in heart, ovary and testis, and almost undetectable in muscle and other tissues. Moreover, the potential involvement of Rspo2 in muscle development was investigated. We found that overexpression of CsRspo2 mRNA in zebrafish embryos resulted in slow development and abnormal muscle formation at the embryonic stage. Our work provides a fundamental understanding of the structure and potential functions of CsRspo2 during muscle development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping