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Fig. 5
Whole-mount in situ hybridization (WISH) of the zebrafish muscle-specific gene MyoD after injection of embryos with CsRspo2 mRNA. (A) Positive signals from antisense probe hybridization are purple or blue. (a, a1) Control group with normally developed embryos at the 11?12-somite stages, (b) embryo injected with CsRspo2 mRNA, and (c) sense probe ISH. (B) Abnormal embryos showing slow development after injection. MyoD mRNA-positive signals are indicated by black arrows. Scale bars = 50 ?m.
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