- Title
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Chemokine C-C motif ligand 33 is a key regulator of teleost fish barbel development
- Authors
- Zhou, T., Li, N., Jin, Y., Zeng, Q., Prabowo, W., Liu, Y., Tian, C., Bao, L., Liu, S., Yuan, Z., Fu, Q., Gao, S., Gao, D., Dunham, R., Shubin, N.H., Liu, Z.
- Source
- Full text @ Proc. Natl. Acad. Sci. USA
Expression of ccl33 genes in zebrafish. (A and B) In situ hybridization of ccl33.2 and ccl33.3 in zebrafish embryos of different developmental stages, including the bud, 1 dpf, 2 dpf, 3 dpf, and 6 dpf. aa, aortic arches; br, brain; ey, eye; e-ysl, external yolk syncytial layer; fb, forebrain; hb, hindbrain; mb, midbrain; mo, mouth; np, neural plate; pfb, pectoral fin bud; pol, polster; tb, tail bud. (C and D) Expression of ccl33.2 and ccl33.3 in adult zebrafish organs using real-time PCR analysis. The y axis represents the relative expression value. Ba, barbel; Fi, caudal fin; Gi, gill; In, intestine; Li, liver; Mo, mouth; Mu, muscle; Sk, skin. |
Knockout of ccl33.2 and ccl33.3 in zebrafish using the CRISPR/Cas9 system. (A) Body length of injected fish and wild-type (WT) fish was not statistically different; however, the barbel length of the injected fish was significantly smaller (P = 0.019) than that of the WT fish. (B) Number of individuals with different phenotypes in the ccl33? group and their associated genotypes. (C) Head region of WT and ccl33? zebrafish at different days after hatching. White arrows indicate the barbels of WT zebrafish. No barbels were observed in the ccl33? individual. (D) Individual with curly barbels in the ccl33? group. dah, days after hatching. (E) Gross morphology of zebrafish from the WT group and the individual with double knockout of ccl33.2 and ccl33.3. PHENOTYPE:
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