Fig. 6
Knockout of ccl33.2 and ccl33.3 in zebrafish using the CRISPR/Cas9 system. (A) Body length of injected fish and wild-type (WT) fish was not statistically different; however, the barbel length of the injected fish was significantly smaller (P = 0.019) than that of the WT fish. (B) Number of individuals with different phenotypes in the ccl33− group and their associated genotypes. (C) Head region of WT and ccl33− zebrafish at different days after hatching. White arrows indicate the barbels of WT zebrafish. No barbels were observed in the ccl33− individual. (D) Individual with curly barbels in the ccl33− group. dah, days after hatching. (E) Gross morphology of zebrafish from the WT group and the individual with double knockout of ccl33.2 and ccl33.3.