Formin antagonism induces tail curving and peripheral tail cell death in developing zebrafish embryos. (A) Representative 2x images at 21 hpt untreated (None) and in 10 μM SMIFH2 (arrow indicates peripheral tail cell death). Scale bar = 2,000 μm. (B–D) Tail degradation was scored for embryos in each untreated, vehicle, and SMIFH2 (B), IMM (C), and PEG-DAD (D) drug conditions. (E-G). Tail curvature was scored for embryos in each untreated, vehicle, and SMIFH2 (E), IMM (F), and PEG-DAD (G) drug condition. The screens were performed in triplicate, five embryos/well and the experiment was repeated three times. p-Value is relative to untreated condition. Error bars indicate SE.

Formin antagonism induces craniofacial defects in developing zebrafish embryos. (A) Representative 2x images at 21 hpt untreated (None) and in 10 μM SMIFH2. (B–D) Craniofacial defects were scored for embryos in each untreated, vehicle, and SMIFH2 (B), IMM (C), and PEG-DAD (D) drug conditions. The screens were performed in triplicate, five embryos/well and the experiment was repeated three times. Values are represented as % expressing squared faces per condition. p- value is relative to untreated condition. Error bars indicate SE. Scale bars = 2,000 μm.

Formin antagonism induces pericardial edema in developing zebrafish embryos. (A) Representative 2x images at 21 hpt without treatment (None) and in 10 μM SMIFH2 (arrow indicates edema). Scale bar = 2,000 μm. (B–D) Pericardial edema was scored for embryos in each untreated, vehicle, and SMIFH2 (B), IMM (C), and PEG-DAD (D) drug conditions. The screens were performed in triplicate, five embryos/well and the experiment was repeated three times. p-value is relative to untreated condition. Error bars indicate SE.