Representative teratology observed from zebrafish embryos treated with 5-aza-dC at 75 µM from 0 to 2 dpf. Teratology was observed from 2 dpf in two out of seven families, and between 3 and 4 dpf, it was observed in all tested families. Different types of teratologies were observed; body curvature, problems with yolk-sac reabsorption and body deformation

Effects of zebrafish treatment with 5-aza-dC at 75 µM during the period of gonadal development (10?30 dpf). (A) External differences between control and 5-aza-dC-treated adult zebrafish females at 90 dpf. Scale in cm. (B) Body weight and (C) standard length of adults at 90 dpf. Data shown as mean ± s.e.m. (n = 8 and 6 males, and 11 and 4 females in control and 5-aza-dC groups, respectively). Within each sex, significant differences (P < 0.05 for males and P < 0.01 for females) in growth were determined by the Student?s t test and are indicated by different letters.