AD0157 inhibits endothelial cell tube formation and migration. (A) BAEC seeded on Matrigel formed tubes. AD0157 inhibited endothelial cell tubulogenesis in vitro in a dose-dependent manner at non-toxic doses. Cells were photographed 7 h after seeding under an inverted microscope (bar = 100 μm); (B) Top panel: confluent BAEC monolayers were wounded, and fresh culture medium was added either in the absence or presence of the indicated concentrations of the compound. Photographs were taken at 7 h of incubation. Broken lines indicate the wound edges (bar = 100 μm); Bottom panel: The regrowth of BAEC into the cell-free area was measured after 7 h, and percentages of the recovered area are expressed as the mean ± SD, * p < 0.05 versus the control (n = 3).

AD0157 inhibits angiogenesis in vivo. (A) Chorioallantoic membrane assay (CAM). The methylcellulose disc containing the substance vehicle alone and the methylcellulose disc containing 0.5, one and 5 nmol of AD0157. Circles show the locations of the methylcellulose discs (bar = 1 mm); (B) Inhibition of the zebrafish neovascularization by AD0157. Transgenic TG(fli1:EGFP)y1 zebrafish embryos that show green fluorescent protein (GFP) expression in endothelial cells were incubated without or with 10 μM AD0157 (bars represent 50 μm).