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Fig. 3
AD0157 inhibits endothelial cell tube formation and migration. (A) BAEC seeded on Matrigel formed tubes. AD0157 inhibited endothelial cell tubulogenesis in vitro in a dose-dependent manner at non-toxic doses. Cells were photographed 7 h after seeding under an inverted microscope (bar = 100 μm); (B) Top panel: confluent BAEC monolayers were wounded, and fresh culture medium was added either in the absence or presence of the indicated concentrations of the compound. Photographs were taken at 7 h of incubation. Broken lines indicate the wound edges (bar = 100 μm); Bottom panel: The regrowth of BAEC into the cell-free area was measured after 7 h, and percentages of the recovered area are expressed as the mean ± SD, * p < 0.05 versus the control (n = 3).