FIGURE SUMMARY
Title

Expression of sclerostin in the developing zebrafish (Danio rerio) brain and skeleton

Authors
McNulty, M.S., Bedell, V.M., Greenwood, T.M., Craig, T.A., Ekker, S.C., and Kumar, R.
Source
Full text @ Gene Expr. Patterns
Fig. 2

Whole mount in situ hybridization of early stage zebrafish embryos (4–12 hpf) using anti-sense or sense Danio rerio sost probe. Panel A. 4 hpf, anti-sense probe. Panel B. 4 hpf, sense probe. Panel C. 6 hpf, anti-sense probe. Panel D. 6 hpf, sense probe. Panel E. 8 hpf, anti-sense probe. Panel F. 8 hpf, sense probe. Panel G. 10 hpf, anti-sense probe. Panel H. 10 hpf, sense probe. Panel I. 12 hpf, anti-sense probe, lateral view Panel J. 12 hpf, sense probe, lateral view. Panel K. 12 hpf, anti-sense probe, dorsal view. Panel L. 12 hpf, sense probe, dorsal view. Panel M. Reverse transcriptase polymerase chain reaction assessment of sost expression and housekeeping gene, 18S. Scale bar = 100 μm.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 2-cell to Day 5
Fig. 3

Whole mount in situ hybridization of zebrafish embryos (15–24 hpf) using anti-sense Danio rerio sost probe. Panel A. 15 hpf, dorsal view. Panel B. 15 hpf, lateral view. Panel C. 18 hpf, dorsal view. Panel D. 18 hpf, lateral view. Panel E. 20 hpf, dorsal view. Panel F. 20 hpf, lateral view. Panel G. 22 hpf, dorsal view. Panel H. 22 hpf, lateral view. Panel I. 24 hpf, dorsal view. Panel J. 24 hpf, lateral view. kV = Kupffer’s vesicle; ERL = early rhombic lip; SC = spinal cord; URL = upper rhombic lip; LRL = lower rhombic lip. Scale bar = 100 μm.
Fig. 4

Whole mount in situ hybridization of zebrafish embryos (28–44 hpf) using anti-sense Danio rerio sost probe. Panel A. 28 hpf, dorsal view. Panel B. 28 hpf, lateral view. Panel C. 32 hpf, dorsal view. Panel D. 32 hpf, lateral view. Panel E. 36 hpf, dorsal view. Panel F. 36 hpf, lateral view. Panel G. 40 hpf, dorsal view. Panel H. 40 hpf, lateral view. Panel I. 44 hpf, dorsal view. Panel J. 44 hpf, lateral view. SC = spinal cord; URL = upper rhombic lip; LRL = lower rhombic lip; PC = pharyngeal cartilage. Scale bar = 100 μm.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to High-pec
Fig. 5

Whole mount in situ hybridization of zebrafish embryos (2–5 dpf) using anti-sense Danio rerio sost probe. Panel A. 2 dpf, dorsal view. Panel B. 2 dpf, lateral view. Panel C. 2 dpf, ventral view. Panel D. 3 dpf, dorsal view. Panel E. 3 dpf, lateral view. Panel F. 3 dpf, ventral view. Panel G. 4 dpf, dorsal view. Panel H. 4 dpf, lateral view. Panel I. 4 dpf, ventral view. Panel J. 5 dpf, dorsal view. Panel K. 5 dpf, lateral view. Panel L. 5 dpf, ventral view. PC = pharyngeal cartilage; PF = pectoral fin; M = Meckel’s; CB = ceratobranchial. Scale bar = 100 μm.
Fig. 6

Whole mount in situ hybridization of zebrafish embryos (3–5 dpf) using anti-sense Danio rerio sost probe. Ventral views. Panel A. 3 dpf. Panel B. 4 dpf. Panel C. 5 dpf. PF = pectoral fin; M = Meckel’s; CB = ceratobranchial. CH = ceratohyal. Scale bar = 200 μm.
Fig. S1

Whole mount in situ hybridization of zebrafish embryos (15 hpf-5 dpf) using sense Danio rerio sost probe. Lateral views. Panel A. 15 hpf. Panel B. 18 hpf. Panel C. 20 hpf. Panel D. 22 hpf. Panel E. 24 hpf. Panel F. 28 hpf. Panel G. 32 hpf. Panel H. 36 hpf. Panel I. 40 hpf. Panel J. 44 hpf. Panel K. 2 dpf. Panel L. 3 dpf. Panel M. 4 dpf. Panel N. 5 dpf. Scale bar = 100 μm.
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Acknowledgments
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Reprinted from Gene expression patterns : GEP, 12(7-8), McNulty, M.S., Bedell, V.M., Greenwood, T.M., Craig, T.A., Ekker, S.C., and Kumar, R., Expression of sclerostin in the developing zebrafish (Danio rerio) brain and skeleton, 228-235, Copyright (2012) with permission from Elsevier. Full text @ Gene Expr. Patterns
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