Mapping Details

GENETIC MAPPING PANELS

Chr	Location	Mapped As	Panel	Mapped By	Scoring
13	43.0 cM	z10582	Boston MGH Cross (MGH)	Fishman, Mark C.	Data
13	2215.0 cR	z10582	Goodfellow T51 (T51)	Geisler, Robert	Data
Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

MAPPING FROM PUBLICATIONS

Marker	Type	Chr	Distance	Publication / Person	Comments
z9289	SSLP	13		Bartman et al., 2004	actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed  ... actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed cfk between ESTs Z9289 and Z10582. This region contained four genes (abcb10, actin, a novel EST fv49b10, and rab4a) that showed conserved synteny with Human chromosome 1q42.13.
acta1b	GENE	13		Bartman et al., 2004	actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed  ... actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed cfk between ESTs Z9289 and Z10582. This region contained four genes (abcb10, actin, a novel EST fv49b10, and rab4a) that showed conserved synteny with Human chromosome 1q42.13.
abcb10	GENE	13		Bartman et al., 2004	actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed  ... actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed cfk between ESTs Z9289 and Z10582. This region contained four genes (abcb10, actin, a novel EST fv49b10, and rab4a) that showed conserved synteny with Human chromosome 1q42.13.
rab4a	GENE	13		Bartman et al., 2004	actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed  ... actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed cfk between ESTs Z9289 and Z10582. This region contained four genes (abcb10, actin, a novel EST fv49b10, and rab4a) that showed conserved synteny with Human chromosome 1q42.13.
z15777	SSLP	13		Kay et al., 2001	Kay et al. (2001. Neuron 30:725-736) report mapping lak th241 to a 0.7 cM region on LG 13 between z15777 and z10582. They also mapped atoh7 to the same region using the T51 radiation hybrid panel. They cloned atoh7 from lak th241 and showed that there was a point mutation, which was confirmed by RFLP analysis.
atoh7	GENE	13	0.71 cM	Kay et al., 2001	Kay et al. (2001. Neuron 30:725-736) report mapping lak th241 to a 0.7 cM region on LG 13 between z15777 and z10582. They also mapped atoh7 to the same region using the T51 radiation hybrid panel. They cloned atoh7 from lak th241 and showed that there was a point mutation, which was confirmed by RFLP analysis.

OTHER MAPPING INFORMATION

Chr 13 Bartman et al., 2004 actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed cfk between ESTs  ... actc (cfk) was mapped to LG13 by bulk-segregant analysis; fine mapping placed cfk between ESTs Z9289 and Z10582. This region contained four genes (abcb10, actin, a novel EST fv49b10, and rab4a) that showed conserved synteny with Human chromosome 1q42.13.

Primer Sets:

Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
AB	245,0,227		60.0
Forward Primer	CCTGTTTGCTGACACTCGAA
Reverse Primer	CACCCCGGTCTCTTAACAAA
IND	0,229		60.0
Forward Primer	CCTGTTTGCTGACACTCGAA
Reverse Primer	CACCCCGGTCTCTTAACAAA
EKW	225,0,227		60.0
Forward Primer	CCTGTTTGCTGACACTCGAA
Reverse Primer	CACCCCGGTCTCTTAACAAA
TU	0,227,237,225		60.0
Forward Primer	CCTGTTTGCTGACACTCGAA
Reverse Primer	CACCCCGGTCTCTTAACAAA