Transgenic Construct
Tg(myo6b:tmie-2A-NLS-mCherry)
- ID
- ZDB-TGCONSTRCT-190820-6
- Name
- Tg(myo6b:tmie-2A-NLS-mCherry)
- Previous Names
-
- Tg(myo6b:tmie-p2A-NLS(mCherry)) (1)
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:mCherry
- Contains Sequences
- None
- Note
-
In this construct, the myo6b promoter drives expression in sensory hair cells of WT tmie coupled to p2A-NLS(mCherry). The p2A linker is a self-cleaving peptide, which leads to translation of equimolar amounts of Tmie and NLS(mCherry). Hence, mCherry expression in the nucleus denotes Tmie expression in the cell.
Plasmid Map
None
Genomic Features
That Utilize tg(myo6b:tmie-2a-nls-mcherry) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| zf2130Tg |
Transgenics
That Utilize tg(myo6b:tmie-2a-nls-mcherry) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| tmieru1000/ru1000; vo27Tg; zf2130Tg (TU) |
Fig. 4
from
Pacentine et al., 2019
|
||
| tmieru1000/ru1000; vo28Tg; zf2130Tg (TU) |
2 figures
from
Pacentine et al., 2019
|
||
| zf2130Tg (TU) |
|
Fig. 4
from
Pacentine et al., 2019
|
Sequence Information
Other Construct Pages
None
Citations