Transgenic Construct

Tg5(Xla.Rho:crb2a-HA)

ID

ZDB-TGCONSTRCT-110131-40

Previous Names

Intra deltaPBD (1)
Tg(Xla.Rho:crb2.5-HA) (1)
Tg(Xla.Rho:crb2a.5-HA)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

crb2a (1)
HA (1)

Contains Sequences

HA (1)

Note

This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide takenfrom zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make the Intra deltaPBD construct, the IntraWT construct was used as a template and site-directed mutagenesis was performed to introduce a Stop codon after the last glutamic acid (E16) in the FERM-binding domain.

Plasmid Map

None

Genomic Features

That Utilize Tg5(Xla.Rho:crb2a-HA) Construct

Genomic Feature	Affected Genomic Regions
umz16Tg
umz17Tg
umz18Tg

Transgenics

That Utilize Tg5(Xla.Rho:crb2a-HA) Construct

Fish	Affected Genomic Regions	Gene Expression
slc45a2^b4/+; fl1Tg/+; umz16Tg/+	slc45a2
slc45a2^b4/+; fl1Tg/+; umz17Tg/+	slc45a2
slc45a2^b4/b4; fl1Tg; umz16Tg	slc45a2	5 figures from Hsu et al., 2010
slc45a2^b4/b4; fl1Tg; umz17Tg	slc45a2
slc45a2^b4/b4; fl1Tg; umz18Tg	slc45a2
umz18Tg

Sequence Information

Other Construct Pages

None

Citations