Transgenic Construct

Tg(atp1a1a.4:EGFP)

ID

ZDB-TGCONSTRCT-070723-1

Name

Tg(atp1a1a.4:EGFP)

Previous Names

Na,K-ATPase alpha1A4:GFP (1)

Type

engineered_plasmid

Regulatory Regions

atp1a1a.4 (1)

Coding Sequences

EGFP (1)

[Em λ][Ex λ]

Fpbase:EGFP

Contains Sequences

None

Note

Liu et al. 2007, ZDB-PUB-070212-38 : The Na,K-ATPase alpha1A4:GFP transgenic line was created by amplifying an 8112 base-pair (bp) fragment of the Na,K- ATPase alpha1A4-subunit promoter (cDNA accession: NM_131689) from genomic DNA using nested PCR with the following primers: LNaK-F1: 5-GGTCGCTGGGCTAAGTCACAGAC-3 LNaK-F2: 5-TGGGCTAAGTCACAGACCGCCTA-3 LNaK-R: 5-TCCACTCACTCCAAGCCCCATTT-3 NaKB-R2: 5-CGCGGATCCCTCACTCCAAGCCCCATTTTCCT-3. The 8112 bp fragment was cloned in pGI upstream of EGFP and injected as a circular plasmid into TuAB wild-type embryos at the 1-cell stage. Founders were screened by GFP fluorescence in the pronephros and were out crossed to create a stable transgenic line.

Plasmid Map

None

Genomic Features

That Utilize Tg(atp1a1a.4:EGFP) Construct

Genomic Feature	Affected Genomic Regions
zf34Tg

Transgenics

That Utilize Tg(atp1a1a.4:EGFP) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
vhl^{hu2117/hu2117}; zf34Tg	vhl	2 figures from Noonan et al., 2016	2 figures from Noonan et al., 2016
zf34Tg			9 figures from 6 publications
zf34Tg + CRISPR1-crkl		Fig. 2 from Lopez-Rivera et al., 2017
zf34Tg + CRISPR1-ret		Fig. 2 from Lopez-Rivera et al., 2017
zf34Tg + CRISPR1-snap29		Fig. 2 from Lopez-Rivera et al., 2017
zf34Tg + MO1-aifm3		Fig. 2 from Lopez-Rivera et al., 2017
zf34Tg + MO1-aifm3 + MO2-snap29		Fig. S7 from Lopez-Rivera et al., 2017
zf34Tg + MO1-camk2g1		Fig. 5 from Rothschild et al., 2011	Fig. 5 from Rothschild et al., 2011
zf34Tg + MO1-crkl		Fig. 2 from Lopez-Rivera et al., 2017
zf34Tg + MO1-lztr1		Fig. S4 from Lopez-Rivera et al., 2017
zf34Tg + MO1-myo1ca		Fig. 5 from Arif et al., 2013	Fig. 5 from Arif et al., 2013
zf34Tg + MO1-myo1cb		Fig. 5 from Arif et al., 2013	Fig. 5 from Arif et al., 2013
zf34Tg + MO1-serpind1		Fig. S4 from Lopez-Rivera et al., 2017
zf34Tg + MO1-slc7a4		Fig. S4 from Lopez-Rivera et al., 2017
zf34Tg + MO2-myo1ca		Fig. 5 from Arif et al., 2013	Fig. 5 from Arif et al., 2013
zf34Tg + MO2-myo1cb		Fig. 5 from Arif et al., 2013	Fig. 5 from Arif et al., 2013
zf34Tg + MO2-snap29		Fig. 2 from Lopez-Rivera et al., 2017
zf34Tg + MO2-yap1		Fig. 3 from He et al., 2015	Fig. 3 from He et al., 2015
zf34Tg + MO3-pkd2		Fig. 8 from Rothschild et al., 2011
zf34Tg + MO3-ret		2 figures from Lopez-Rivera et al., 2017
zf34Tg + MO5-pi4kaa		Fig. S4 from Lopez-Rivera et al., 2017
zf34Tg/+

Sequence Information

Other Construct Pages

None

Citations