TALEN

TALEN1-dgcr8

ID

ZDB-TALEN-180718-1

Name

TALEN1-dgcr8

Previous Names

None

Target

dgcr8 (1)

Target Sequence 1

5' - TAGCACAAAAACCGAAAC - 3'

Target Sequence 2

5' - TTGTAATTGTTTAATTGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf1064	dgcr8

Expression

Gene expression in Wild Types + TALEN1-dgcr8

No data available

Phenotype

Phenotype resulting from TALEN1-dgcr8

No data available

Phenotype of all Fish created by or utilizing TALEN1-dgcr8

Phenotype	Fish	Conditions	Figures
whole organism decreased size, abnormal	dgcr8^{zf1064/zf1064}	standard conditions	Fig. 1 from Liu et al., 2017
whole organism decreased life span, abnormal	dgcr8^{zf1064/zf1064}	standard conditions	Fig. 1 from Liu et al., 2017
hindbrain mitotic cell cycle decreased occurrence, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 3 from Zhu et al., 2020
yolk syncytial layer erythroblast absent, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 5 from Zhu et al., 2020
cardiac muscle myoblast myl7 expression mislocalised, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 4 from Zhu et al., 2020
cardiac muscle myoblast mislocalized abaxially, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 4 from Zhu et al., 2020
heart malformed, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 , FIGURE 4 from Zhu et al., 2020
thigmotaxis decreased occurrence, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 5 from Zhu et al., 2020
hatching decreased occurrence, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 5 from Zhu et al., 2020
brain malformed, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 from Zhu et al., 2020
heart contraction decreased frequency, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 4 from Zhu et al., 2020
epiboly involved in gastrulation with mouth forming second disrupted, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 from Zhu et al., 2020
whole organism curved, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 from Zhu et al., 2020
caudal vein erythroblast mislocalised, abnormal	dgcr8^zf1064/+; ihb7Tg; zf1065Tg	standard conditions	FIGURE 5 from Zhu et al., 2020
ovary kiss2 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
ovary sox9a expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
ovary gdf9 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
ovary lhcgr expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
ovary fshr expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
ovary dre-mir-21-1 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
ovary gsdf expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
ovary inha expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
ovary ddx4 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg/+; zf1065Tg/+	standard conditions	FIGURE 1 from Zhu et al., 2020
blood circulation arrested, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 5 from Liu et al., 2017
whole organism sh2d5 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism zgc:171776 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
cardiac muscle myoblast mislocalized abaxially, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 4 from Zhu et al., 2020
whole organism hnrnpa0a expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
hatching decreased occurrence, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 5 from Zhu et al., 2020
whole organism si:dkey-29j8.3 expression absent, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism bicc1a expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
brain decreased size, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 5 from Liu et al., 2017
endoderm sox17 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 6 from Liu et al., 2017
midbrain hindbrain boundary neural rod pax2a expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 6 from Liu et al., 2017
whole organism ly75 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
presumptive dorsal mesoderm chrd expression decreased distribution, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 6 from Liu et al., 2017
whole organism si:ch211-263m18.4 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism amt expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism abcd3a expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism eif4ebp2 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism znf1053 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism dnm1l expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism ddx1 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism cd82b expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
thigmotaxis decreased occurrence, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 5 from Zhu et al., 2020
whole organism tdrd1 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism si:dkey-19a16.7 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
presumptive dorsal mesoderm gsc expression decreased distribution, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 6 from Liu et al., 2017
whole organism tdrd6 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism dock4b expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
myeloid cell spi1b expression absent, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 6 from Liu et al., 2017
whole organism akr1b1.2 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
primary miRNA processing decreased occurrence, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 4 from Liu et al., 2017
whole organism smarca2 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism si:dkey-21a6.5 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism ago2 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
embryo development delayed, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 5 from Liu et al., 2017
whole organism si:dkey-122c11.4 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism itgam.1 expression absent, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism si:ch1073-190k2.1 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism si:ch211-209p16.1 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism pho expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism phc2b expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
heart contraction decreased frequency, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 4 from Zhu et al., 2020
brain morphology, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 5 from Liu et al., 2017
whole organism opa1 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism zgc:152891 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism mir25 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 4 from Liu et al., 2017
whole organism zgc:163014 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism ppt2a.4 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism sbf2 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
yolk syncytial layer erythroblast absent, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 5 from Zhu et al., 2020
whole organism ndrg1b expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism hsp90aa1.2 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism si:dkey-119f1.1 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism si:ch211-259g3.4 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism selenoh expression absent, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism dnajc5ga expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism mt-nd6 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
caudal vein erythroblast mislocalised, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 5 from Zhu et al., 2020
heart malformed, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 , FIGURE 4 from Zhu et al., 2020
whole organism znf997 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism foxd3 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism zgc:113208 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism curved, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 from Zhu et al., 2020
cardioblast migration to the midline involved in heart field formation decreased occurrence, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 6 from Liu et al., 2017
endoderm sox32 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 6 from Liu et al., 2017
epiboly process quality, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 5 from Liu et al., 2017
whole organism dre-mir-21-1 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 4 from Liu et al., 2017
whole organism znf1063 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
heart contraction arrested, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 5 from Liu et al., 2017
whole organism dicer1 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
cardiac muscle myoblast myl7 expression mislocalised, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 4 from Zhu et al., 2020
whole organism znf1031 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism buc expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
miRNA-mediated post-transcriptional gene silencing decreased occurrence, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 4 from Liu et al., 2017
epiboly involved in gastrulation with mouth forming second disrupted, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 from Zhu et al., 2020
whole organism hsp70.3 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism mtus1a expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism akap13 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
hindbrain mitotic cell cycle decreased occurrence, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 3 from Zhu et al., 2020
whole organism acadl expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism zgc:173709 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism viability, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 from Zhu et al., 2020
otic placode pax2a expression spatial pattern, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 6 from Liu et al., 2017
brain malformed, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 2 from Zhu et al., 2020
whole organism si:ch1073-263o8.2 expression decreased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism plcb3 expression increased amount, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	FIGURE 7 from Zhu et al., 2020
whole organism anterior-posterior axis increased length, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 5 from Liu et al., 2017
whole organism dorsal-ventral axis decreased size, abnormal	dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg	standard conditions	Fig. 5 from Liu et al., 2017

Citations