FIGURE

FIGURE 5

ID

ZDB-FIG-200423-59

Publication

Zhu et al., 2020 - Functional Characterization and Expression Analyses Show Differential Roles of Maternal and Zygotic Dgcr8 in Early Embryonic Development

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FIGURE 5

Maternal mutants exhibit reduced erythropoiesis and could be rescued in Mdgcr8, but not in MZdgcr8. Whole embryo O-dianisidine staining assay was used for assessing functional hemoglobin in mature primitive erythrocytes in wild-type, Mdgcr8, MZdgcr8, and miR-430 rescued groups at 50 (A) and 74 hpf (B). Hemoglobinized cells accumulate in the yolk sac in wild-type but mainly accumulate in the caudal vein (red arrows) in MZdgcr8 and MZdgcr8 mutants. 10–15 embryos per groups. Scale bar: 200 μm. (C) The touch response was categorized as strong, weak, and no response based on the analysis of the video frames. (D) At 60 hpf, the rescue rate was determined in wild-type and maternal mutant embryos. (E) At 48 hpf, the hatching rate was detected in wild-type and maternal mutant embryos. ****p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dgcr8^zf1064/+; ihb7Tg; zf1065Tg dgcr8^{zf1064/zf1064}; ihb7Tg; zf1065Tg
Observed In:	caudal vein erythroblast hatching thigmotaxis yolk syncytial layer erythroblast
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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