PUBLICATION
Isolation of the zebrafish homologues for the tie-1 and tie-2 endothelium-specific receptor tyrosine kinases
- Authors
- Lyons, M.S., Bell, B., Stainier, D., and Peters, K.G.
- ID
- ZDB-PUB-980519-2
- Date
- 1998
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 212(1): 133-140 (Journal)
- Registered Authors
- Lyons, Michael Steffen, Peters, Kevin G., Stainier, Didier
- Keywords
- zebrafish; endothelium; receptor tyrosine kinase; blood vessel
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary
- Endothelium, Vascular/embryology
- Endothelium, Vascular/enzymology*
- Gene Expression
- Humans
- In Situ Hybridization
- Molecular Sequence Data
- Receptor Protein-Tyrosine Kinases/genetics*
- Receptor, TIE-1
- Receptor, TIE-2
- Receptors, Cell Surface/genetics*
- Receptors, TIE
- Sequence Homology, Amino Acid
- Zebrafish
- Zebrafish Proteins*
- PubMed
- 9603430 Full text @ Dev. Dyn.
Citation
Lyons, M.S., Bell, B., Stainier, D., and Peters, K.G. (1998) Isolation of the zebrafish homologues for the tie-1 and tie-2 endothelium-specific receptor tyrosine kinases. Developmental Dynamics : an official publication of the American Association of Anatomists. 212(1):133-140.
Abstract
Several characteristics of the zebrafish embryo make it an attractive model in which to study the development of the cardiovascular system. The utility of the zebrafish as a model of mammalian vascular development will depend on the conservation of molecular and morphogenetic mechanisms of vessel growth. Here, we report the cloning of the zebrafish homologues of the endothelium-specific receptor tyrosine kinases tie-1 and tie-2. The Z tie-2 clone represents the first report of a full-length zebrafish endothelium-specific gene. The zebrafish tie family members have significant structural homology with their murine and human counterparts. In addition, like the murine tie-1 and tie-2 genes, expression was found predominantly in endothelial cells. At 24-hr postfertilization (HPF), Z tie-1 was expressed in all observed populations of endothelial cells. Interestingly, Z tie-2 exhibited a similar, although slightly more restricted, expression pattern. Taken together, these data strongly suggest that mechanisms of vascular development are highly conserved across species and that zebrafish will continue to be a useful model for the investigation of vertebrate embryonic vascular development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping