ZFIN ID: ZDB-PUB-980519-2
Isolation of the zebrafish homologues for the tie-1 and tie-2 endothelium-specific receptor tyrosine kinases
Lyons, M.S., Bell, B., Stainier, D., and Peters, K.G.
Date: 1998
Source: Developmental dynamics : an official publication of the American Association of Anatomists 212(1): 133-140 (Journal)
Registered Authors: Lyons, Michael Steffen, Peters, Kevin G., Stainier, Didier
Keywords: zebrafish; endothelium; receptor tyrosine kinase; blood vessel
MeSH Terms: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Complementary (all 19) expand
PubMed: 9603430 Full text @ Dev. Dyn.
FIGURES   (current status)
Several characteristics of the zebrafish embryo make it an attractive model in which to study the development of the cardiovascular system. The utility of the zebrafish as a model of mammalian vascular development will depend on the conservation of molecular and morphogenetic mechanisms of vessel growth. Here, we report the cloning of the zebrafish homologues of the endothelium-specific receptor tyrosine kinases tie-1 and tie-2. The Z tie-2 clone represents the first report of a full-length zebrafish endothelium-specific gene. The zebrafish tie family members have significant structural homology with their murine and human counterparts. In addition, like the murine tie-1 and tie-2 genes, expression was found predominantly in endothelial cells. At 24-hr postfertilization (HPF), Z tie-1 was expressed in all observed populations of endothelial cells. Interestingly, Z tie-2 exhibited a similar, although slightly more restricted, expression pattern. Taken together, these data strongly suggest that mechanisms of vascular development are highly conserved across species and that zebrafish will continue to be a useful model for the investigation of vertebrate embryonic vascular development.