PUBLICATION

Implications for dorsoventral axis determination from the zebrafish mutation janus

Authors
Abdelilah, S., Solnica-Krezel, L., Stainier, D.Y.R., and Driever, W.
ID
ZDB-PUB-961014-12
Date
1994
Source
Nature   370: 468-471 (Journal)
Registered Authors
Abdelilah-Seyfried, Salim, Driever, Wolfgang, Solnica-Krezel, Lilianna, Stainier, Didier
Keywords
none
MeSH Terms
  • Animals
  • Blastoderm/cytology*
  • Cell Polarity
  • DNA-Binding Proteins/biosynthesis
  • Embryo, Nonmammalian/metabolism
  • Genes, Recessive
  • Goosecoid Protein
  • Homeodomain Proteins*
  • Mutation*
  • Repressor Proteins*
  • Temperature
  • Transcription Factors*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins
PubMed
8047167 Full text @ Nature
Abstract
THE mechanisms underlying the formation of dorsoventral polarity in the zebrafish Danio rerio are unknown. Here we describe the zebrafish recessive maternal-effect mutation janusm55. The mutant phenotype is a division of the blastoderm along the first cleavage plane into two detached half-sized blastoderms. Partial-axis bifurcation occurs in a subset of mutants. Analysis of goosecoid expression in the mutant embryos indicates that only one organizer region is present in each embryo. Furthermore, the position of this organizer region is random with respect to the first cleavage plane bisecting the two blastoderms. Finally, cell tracing in wild-type embryos demonstrates that there is no strict correlation of the dorsoventral axis with early cleavage planes in zebrafish. These findings support the notion that the establishment of the dorsoventral axis and the first cleavage planes are determined by separate mechanisms in the zebrafish embryo.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping