PUBLICATION
The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion
- Authors
- Hollander-Cohen, L., Cohen, O., Shulman, M., Aiznkot, T., Fontanaud, P., Revah, O., Mollard, P., Golan, M., Levavi-Sivan, B.
- ID
- ZDB-PUB-250109-42
- Date
- 2024
- Source
- eLIFE 13: (Journal)
- Registered Authors
- Golan, Matan, Levavi-Sivan, Berta
- Keywords
- CCK, fsh, neuroscience, pituitary, reproduction, zebrafish
- MeSH Terms
-
- Gonadotropin-Releasing Hormone/genetics
- Gonadotropin-Releasing Hormone/metabolism
- Zebrafish*/metabolism
- Zebrafish*/physiology
- Gonadotropins/metabolism
- Follicle Stimulating Hormone/metabolism
- Reproduction*
- Animals
- Female
- Luteinizing Hormone/metabolism
- Cholecystokinin*/metabolism
- PubMed
- 39717899 Full text @ Elife
Citation
Hollander-Cohen, L., Cohen, O., Shulman, M., Aiznkot, T., Fontanaud, P., Revah, O., Mollard, P., Golan, M., Levavi-Sivan, B. (2024) The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion. eLIFE. 13:.
Abstract
Life histories of oviparous species dictate high metabolic investment in the process of gonadal development leading to ovulation. In vertebrates, these two distinct processes are controlled by the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH), respectively. While it was suggested that a common secretagogue, gonadotropin-releasing hormone (GnRH), oversees both functions, the generation of loss-of-function fish challenged this view. Here, we reveal that the satiety hormone cholecystokinin (CCK) is the primary regulator of this axis in zebrafish. We found that FSH cells express a CCK receptor, and our findings demonstrate that mutating this receptor results in a severe hindrance to ovarian development. Additionally, it causes a complete shutdown of both gonadotropins secretion. Using in-vivo and ex-vivo calcium imaging of gonadotrophs, we show that GnRH predominantly activates LH cells, whereas FSH cells respond to CCK stimulation, designating CCK as the bona fide FSH secretagogue. These findings indicate that the control of gametogenesis in fish was placed under different neural circuits, that are gated by CCK.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping