PUBLICATION
Piezo 1 and Piezo 2 in the Chemosensory Organs of Zebrafish (Danio rerio)
- Authors
- Aragona, M., Mhalhel, K., Cometa, M., Franco, G.A., Montalbano, G., Guerrera, M.C., Levanti, M., Laurà, R., Abbate, F., Vega, J.A., Germanà, A.
- ID
- ZDB-PUB-240713-16
- Date
- 2024
- Source
- International Journal of Molecular Sciences 25(13): (Journal)
- Registered Authors
- Aragona, Maria Luisa, Cometa, Marzio, Germanà, Antonino, Guerrera, Maria Cristina, Mhalhel, Kamel, Montalbano, Giuseppe
- Keywords
- Piezo 1, Piezo 2, isolated chemosensory cells, olfactory epithelium, sensory organ, taste buds, translational medicine, zebrafish
- MeSH Terms
-
- Animals
- Calbindin 2/metabolism
- Ion Channels*/genetics
- Ion Channels*/metabolism
- Olfactory Mucosa/metabolism
- Taste Buds/metabolism
- Zebrafish*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 39000511 Full text @ Int. J. Mol. Sci.
Citation
Aragona, M., Mhalhel, K., Cometa, M., Franco, G.A., Montalbano, G., Guerrera, M.C., Levanti, M., Laurà, R., Abbate, F., Vega, J.A., Germanà, A. (2024) Piezo 1 and Piezo 2 in the Chemosensory Organs of Zebrafish (Danio rerio). International Journal of Molecular Sciences. 25(13):.
Abstract
The ion channels Piezo 1 and Piezo 2 have been identified as membrane mechano-proteins. Studying mechanosensitive channels in chemosensory organs could help in understanding the mechanisms by which these channels operate, offering new therapeutic targets for various disorders. This study investigates the expression patterns of Piezo proteins in zebrafish chemosensory organs. For the first time, Piezo protein expression in adult zebrafish chemosensory organs is reported. In the olfactory epithelium, Piezo 1 immunolabels kappe neurons, microvillous cells, and crypt neurons, while Calretinin is expressed in ciliated sensory cells. The lack of overlap between Piezo 1 and Calretinin confirms Piezo 1's specificity for kappe neurons, microvillous cells, and crypt neurons. Piezo 2 shows intense immunoreactivity in kappe neurons, one-ciliated sensory cells, and multi-ciliated sensory cells, with overlapping Calretinin expression, indicating its olfactory neuron nature. In taste buds, Piezo 1 immunolabels Merkel-like cells at the bases of cutaneous and pharyngeal taste buds and the light and dark cells of cutaneous and oral taste buds. It also marks the dark cells of pharyngeal taste buds and support cells in oral taste buds. Piezo 2 is found in the light and dark cells of cutaneous and oral taste buds and isolated chemosensory cells. These findings provide new insights into the distribution of Piezo channels in zebrafish chemosensory organs, enhancing our understanding of their sensory processing and potential therapeutic applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping