PUBLICATION
Deciphering the function of the fifth class of Gα proteins: regulation of ionic homeostasis as unifying hypothesis
- Authors
- Abu Obaid, A., Ivandic, I., Korsching, S.I.
- ID
- ZDB-PUB-240511-4
- Date
- 2024
- Source
- Cellular and molecular life sciences : CMLS 81: 213213 (Journal)
- Registered Authors
- Korsching, Sigrun
- Keywords
- G protein-coupled receptor, Gnav, Oogenesis, Osmoregulation, Signal transduction
- MeSH Terms
-
- Kidney/metabolism
- Animals
- GTP-Binding Protein alpha Subunits/genetics
- GTP-Binding Protein alpha Subunits/metabolism
- Homeostasis*/genetics
- PubMed
- 38727814 Full text @ Cell. Mol. Life Sci.
Abstract
Trimeric G proteins transduce signals from a superfamily of receptors and each G protein controls a wide range of cellular and systemic functions. Their highly conserved alpha subunits fall in five classes, four of which have been well investigated (Gs, Gi, G12, Gq). In contrast, the function of the fifth class, Gv is completely unknown, despite its broad occurrence and evolutionary ancient origin (older than metazoans). Here we show a dynamic presence of Gv mRNA in several organs during early development of zebrafish, including the hatching gland, the pronephros and several cartilage anlagen, employing in situ hybridisation. Next, we generated a Gv frameshift mutation in zebrafish and observed distinct phenotypes such as reduced oviposition, premature hatching and craniofacial abnormalities in bone and cartilage of larval zebrafish. These phenotypes could suggest a disturbance in ionic homeostasis as a common denominator. Indeed, we find reduced levels of calcium, magnesium and potassium in the larvae and changes in expression levels of the sodium potassium pump atp1a1a.5 and the sodium/calcium exchanger ncx1b in larvae and in the adult kidney, a major osmoregulatory organ. Additionally, expression of sodium chloride cotransporter slc12a3 and the anion exchanger slc26a4 is altered in complementary ways in adult kidney. It appears that Gv may modulate ionic homeostasis in zebrafish during development and in adults. Our results constitute the first insight into the function of the fifth class of G alpha proteins.
Genes / Markers
Figure Gallery (6 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping