PUBLICATION

Calcium imaging of adult olfactory epithelium reveals amines as important odor class in fish

Authors
Dieris, M., Kowatschew, D., Hassenklöver, T., Manzini, I., Korsching, S.I.
ID
ZDB-PUB-240213-7
Date
2024
Source
Cell and tissue research   396(1): 95-102 (Journal)
Registered Authors
Korsching, Sigrun
Keywords
Amine, Ciliated neuron, Odorant, Olfaction, Zebrafish
MeSH Terms
  • Amines/metabolism
  • Animals
  • Calcium/metabolism
  • Odorants
  • Olfactory Mucosa/metabolism
  • Olfactory Receptor Neurons*/metabolism
  • Receptors, Odorant*/metabolism
  • Zebrafish/metabolism
PubMed
38347202 Full text @ Cell Tissue Res.
Abstract
The odor space of aquatic organisms is by necessity quite different from that of air-breathing animals. The recognized odor classes in teleost fish include amino acids, bile acids, reproductive hormones, nucleotides, and a limited number of polyamines. Conversely, a significant portion of the fish olfactory receptor repertoire is composed of trace amine-associated receptors, generally assumed to be responsible for detecting amines. Zebrafish possess over one hundred of these receptors, but the responses of olfactory sensory neurons to amines have not been known so far. Here we examined odor responses of zebrafish olfactory epithelial explants at the cellular level, employing calcium imaging. We report that amines elicit strong responses in olfactory sensory neurons, with a time course characteristically different from that of ATP-responsive (basal) cells. A quantitative analysis of the laminar height distribution shows amine-responsive cells undistinguishable from ciliated neurons positive for olfactory marker protein. This distribution is significantly different from those measured for microvillous neurons positive for transient receptor potential channel 2 and basal cells positive for proliferating cell nuclear antigen. Our results suggest amines as an important odor class for teleost fish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping