PUBLICATION
Methods for dynamic and whole volume imaging of the zebrafish heart
- Authors
- Bakis, I., Sun, Y., Abd Elmagid, L., Feng, X., Garibyan, M., Yip, J., Yu, F.Z., Chowdhary, S., Fernandez, E., Cao, J., McCain, M., Lien, C.L., Harrison, M.R.
- ID
- ZDB-PUB-230915-55
- Date
- 2023
- Source
- Developmental Biology 504: 75-85 (Journal)
- Registered Authors
- Cao, Jingli, Harrison, Michael, Lien, Ching-Ling (Ellen)
- Keywords
- Antibody labeling, Clearing, Coronary vessels, Imaging, Live-cell imaging, Regeneration, Zebrafish
- MeSH Terms
-
- Animals
- Endothelial Cells*/metabolism
- Heart/diagnostic imaging
- Imaging, Three-Dimensional/methods
- Zebrafish*
- PubMed
- 37708968 Full text @ Dev. Biol.
Citation
Bakis, I., Sun, Y., Abd Elmagid, L., Feng, X., Garibyan, M., Yip, J., Yu, F.Z., Chowdhary, S., Fernandez, E., Cao, J., McCain, M., Lien, C.L., Harrison, M.R. (2023) Methods for dynamic and whole volume imaging of the zebrafish heart. Developmental Biology. 504:75-85.
Abstract
Tissue development and regeneration are dynamic processes involving complex cell migration and cell-cell interactions. We have developed a protocol for complementary time-lapse and three-dimensional (3D) imaging of tissue for developmental and regeneration studies which we apply here to the zebrafish cardiac vasculature. 3D imaging of fixed specimens is used to first define the subject at high resolution then live imaging captures how it changes dynamically. Hearts from adult and juvenile zebrafish are extracted and cleaned in preparation for the different imaging modalities. For whole-mount 3D confocal imaging, single or multiple hearts with native fluorescence or immuno-labeling are prepared for stabilization or clearing, and then imaged. For live imaging, hearts are placed in a prefabricated fluidic device and set on a temperature-controlled microscope for culture and imaging over several days. This protocol allows complete visualization of morphogenic processes in a 3D context and provides the ability to follow cell behaviors to complement in vivo and fixed tissue studies. This culture and imaging protocol can be applied to different cell and tissue types. Here, we have used it to observe zebrafish coronary vasculature and the migration of coronary endothelial cells during heart regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping