ZFIN ID: ZDB-PERS-070105-1
Lien, Ching-Ling (Ellen)
Email: clien@chla.usc.edu
URL: http://www.usc.edu/programs/pibbs/site/faculty/lien_c.htm
Affiliation: Lien Lab
Address: The Saban Research Institute Childrens Hospital Los Angeles 4661 Sunset Blvd. MS#137 Los Angeles, CA 90027 USA
Country: United States
Phone: 323-644-8377


Bakis, I., Sun, Y., Abd Elmagid, L., Feng, X., Garibyan, M., Yip, J., Yu, F.Z., Chowdhary, S., Fernandez, E., Cao, J., McCain, M., Lien, C.L., Harrison, M.R. (2023) Methods for dynamic and whole volume imaging of the zebrafish heart. Developmental Biology. 504:75-85
Travisano, S.I., Harrison, M.R.M., Thornton, M.E., Grubbs, B.H., Quertermous, T., Lien, C.L. (2023) Single-nuclei multiomic analyses identify human cardiac lymphatic endothelial cells associated with coronary arteries in the epicardium. Cell Reports. 42:113106113106
Sun, Y., Kumar, S.R., Wong, C.E.D., Tian, Z., Bai, H., Crump, J.G., Bajpai, R., Lien, C.L. (2022) Craniofacial and cardiac defects in chd7 zebrafish mutants mimic CHARGE syndrome. Frontiers in cell and developmental biology. 10:10305871030587
Abd Elmagid, L., Mittal, N., Bakis, I., Lien, C.L., Harrison, M.R. (2022) Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish. Journal of visualized experiments : JoVE. (187):
Kapuria, S., Bai, H., Fierros, J., Huang, Y., Ma, F., Yoshida, T., Aguayo, A., Kok, F., Wiens, K.M., Yip, J.K., McCain, M.L., Pellegrini, M., Nagashima, M., Hitchcock, P.F., Mochizuki, N., Lawson, N.D., Harrison, M.M.R., Lien, C.L. (2022) Heterogeneous pdgfrβ+ cells regulate coronary vessel development and revascularization during heart regeneration. Development (Cambridge, England). 149(4):
Shen, H., Darehzereshki, A., Sucov, H.M., Lien, C.L. (2021) Apical Resection and Cryoinjury of Neonatal Mouse Heart. Methods in molecular biology (Clifton, N.J.). 2158:23-32
Yip, J.K., Harrison, M., Villafuerte, J., Fernandez, G.E., Petersen, A.P., Lien, C.L., McCain, M.L. (2019) Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device. Lab on a Chip. 20(2):274-284
Harrison, M.R., Feng, X., Mo, G., Aguayo, A., Villafuerte, J., Yoshida, T., Pearson, C.A., Schulte-Merker, S., Lien, C.L. (2019) Late developing cardiac lymphatic vasculature supports adult zebrafish heart function and regeneration. eLIFE. 8:
Yeo, S., Yoon, C., Lien, C.L., Song, T.K., Shung, K.K. (2019) Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging. Sensors (Basel, Switzerland). 19(19):
Kapuria, S., Yoshida, T., Lien, C.L. (2018) Coronary Vasculature in Cardiac Development and Regeneration. Journal of cardiovascular development and disease. 5(4):
Li, Z., Tyrpak, D.R., MacKay, J.A., Lien, C.L. (2018) Tunable assembly of protein-microdomains in living vertebrate embryos. Advanced biosystems. 2(10):
Patterson, M., Barske, L., Van Handel, B., Rau, C.D., Gan, P., Sharma, A., Parikh, S., Denholtz, M., Huang, Y., Yamaguchi, Y., Shen, H., Allayee, H., Crump, J.G., Force, T.I., Lien, C.L., Makita, T., Lusis, A.J., Kumar, S.R., Sucov, H.M. (2017) Frequency of mononuclear diploid cardiomyocytes underlies natural variation in heart regeneration. Nature Genetics. 49(9):1346-1353
Schall, K.A., Holoyda, K.A., Isani, M., Schlieve, C., Salisbury, T., Khuu, T., Debelius, J.W., Moats, R.A., Pollack, H.A., Lien, C.L., Fowler, K., Hou, X., Knight, R., Grikscheit, T.C. (2017) Intestinal adaptation in proximal and distal segments: Two epithelial responses diverge after intestinal separation. Surgery. 161(4):1016-1027
Schall, K.A., Holoyda, K.A., Isani, M., Lien, C.L., Al Alam, D., Grikscheit, T.C. (2017) Inhibition of Fgf signaling in short bowel syndrome increases weight loss and epithelial proliferation. Surgery. 161(3):694-703
Gamba, L., Amin-Javaheri, A., Kim, J., Warburton, D., Lien, C.L. (2017) Collagenolytic Activity Is Associated with Scar Resolution in Zebrafish Hearts after Cryoinjury. Journal of cardiovascular development and disease. 4(1)
Schall, K.A., Thornton, M.E., Isani, M., Holoyda, K.A., Hou, X., Lien, C.L., Grubbs, B.H., Grikscheit, T.C. (2017) Short bowel syndrome results in increased gene expression associated with proliferation, inflammation, bile acid synthesis and immune system activation: RNA sequencing a zebrafish SBS model. BMC Genomics. 18:23
Miguel, J.C., Maxwell, A.A., Hsieh, J.J., Harnisch, L.C., Al Alam, D., Polk, D.B., Lien, C.L., Watson, A.J., Frey, M.R. (2017) Epidermal growth factor suppresses intestinal epithelial cell shedding via a MAPK dependent pathway. Journal of Cell Science. 130(1):90-96
Rubin, N., Harrison, M.R., Krainock, M., Kim, R., Lien, C.L. (2016) Recent advancements in understanding endogenous heart regeneration- insights from adult zebrafish and neonatal mice. Seminars in cell & developmental biology. 58:34-40
Harrison, M.R., Bussmann, J., Huang, Y., Zhao, L., Osorio, A., Burns, C.G., Burns, C.E., Sucov, H.M., Siekmann, A.F., Lien, C.L. (2015) Chemokine-guided angiogenesis directs coronary vasculature formation in zebrafish. Developmental Cell. 33:442-54
Kang, B.J., Park, J., Kim, J., Kim, H.H., Lee, C., Hwang, J.Y., Lien, C.L., Shung, K.K. (2015) High-frequency dual mode pulsed wave Doppler imaging for monitoring the functional regeneration of adult zebrafish hearts. Journal of the Royal Society, Interface. 12(103):2968-74
Li, R., Beebe, T., Jen, N., Yu, F., Takabe, W., Harrison, M., Cao, H., Lee, J., Yang, H., Han, P., Wang, K., Shimizu, H., Chen, J., Lien, C.L., Chi, N.C., Hsiai, T.K. (2014) Shear Stress-Activated Wnt-Angiopoietin-2 Signaling Recapitulates Vascular Repair in Zebrafish Embryos. Arterioscler. Thromb. Vasc. Biol.. 34(10):2268-75
Gamba, L., Harrison, M., and Lien, C.L. (2014) Cardiac regeneration in model organisms. Current Treatment Options in Cardiovascular Medicine. 16(3):288
Lee, J., Moghadam, M.E., Kung, E., Cao, H., Beebe, T., Miller, Y., Roman, B.L., Lien, C.L., Chi, N.C., Marsden, A.L., and Hsiai, T.K. (2013) Moving domain computational fluid dynamics to interface with an embryonic model of cardiac morphogenesis. PLoS One. 8(8):e72924
Huang, Y., Harrison, M.R., Osorio, A., Kim, J., Baugh, A., Duan, C., Sucov, H.M., and Lien, C.L. (2013) Igf Signaling is Required for Cardiomyocyte Proliferation during Zebrafish Heart Development and Regeneration. PLoS One. 8(6):e67266
Park, J., Cummins, T.M., Harrison, M., Lee, J., Zhou, Q., Lien, C.L., and Shung, K.K. (2013) High frequency photoacoustic imaging for in vivo visualizing blood flow of zebrafish heart. Optics express. 21(12):14636-14642
Park, J., Huang, Y., Chen, R., Lee, J., Cummins, T.M., Zhou, Q., Lien, C.L., and Shung, K.K. (2013) Pulse Inversion Chirp Coded Tissue Harmonic Imaging (PI-CTHI) of Zebrafish Heart Using High Frame Rate Ultrasound Biomicroscopy. Annals of biomedical engineering. 41(1):41-52
Lien, C.L., Harrison, M.R., Tuan, T.L., and Starnes, V.A. (2012) Heart repair and regeneration: Recent insights from zebrafish studies. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. 20(5):638-646
Kim, J., Rubin, N., Huang, Y., Tuan, T.L., and Lien, C.L. (2012) In vitro culture of epicardial cells from adult zebrafish heart on a fibrin matrix. Nature Protocols. 7(2):247-255
Kim, J., Wu, Q., Zhang, Y., Wiens, K.M., Huang, Y., Rubin, N., Shimada, H., Handin, R.I., Chao, M.Y., Tuan, T.L., Starnes, V.A., and Lien, C.L. (2010) PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts. Proceedings of the National Academy of Sciences of the United States of America. 107(40):17206-17210
Wiens, K.M., Lee, H.L., Shimada, H., Metcalf, A.E., Chao, M.Y., and Lien, C.L. (2010) Platelet-derived growth factor receptor Beta is critical for zebrafish intersegmental vessel formation. PLoS One. 5(6):e11324
Sun, P., Zhang, Y., Yu, F., Parks, E., Lyman, A., Wu, Q., Ai, L., Hu, C.H., Zhou, Q., Shung, K., Lien, C.L., and Hsiai, T.K. (2009) Micro-Electrocardiograms to Study Post-Ventricular Amputation of Zebrafish Heart. Annals of biomedical engineering. 37(5):890-901
Lien, C.L., Schebesta, M., Makino, S., Weber, G.J., and Keating, M.T. (2006) Gene Expression Analysis of Zebrafish Heart Regeneration. PLoS Biology. 4(8):e260
Schebesta, M., Lien, C.L., Engel, F.B., and Keating, M.T. (2006) Transcriptional profiling of caudal fin regeneration in zebrafish. TheScientificWorldJournal. 6(1):38-54
Whitehead, G.G., Makino, S., Lien, C.L., and Keating, M.T. (2005) fgf20 is essential for initiating zebrafish fin regeneration. Science (New York, N.Y.). 310(5756):1957-1960
Makino, S., Whitehead, G.G., Lien, C.L., Kim, S., Jhawar, P., Kono, A., Kawata, Y., and Keating, M.T. (2005) Heat-shock protein 60 is required for blastema formation and maintenance during regeneration. Proceedings of the National Academy of Sciences of the United States of America. 102(41):14599-14604

1. Wu SM, Fujiwara Y, Cibulsky SM, Clapham DE, Lien CL, Schultheiss TM, Orkin SH. Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart.
Cell. 15: 1137-1159 2006.

2. Xin M, Davis CA, Molkentin JD, Lien CL, Duncan SA, Richardson JA, Olson EN.
A threshold of GATA4 and GATA6 expression is required for cardiovascular development.
Proc. Natl. Acad. Sci. U. S. A.103: 11189-94, 2006.

3. Oh J, Wang Z, Wang DZ, Lien CL, Zing W, Olson EN.
Target gene-specific modulation of myocardin activity by GATA transcription factors. Mol Cell Biol. 24: 8519-28, 2004.

4. Lien CL, McAnally J, Richardson JA, Olson EN.
Cardiac-specific activity of an Nkx2-5 enhancer requires an evolutionarily conserved Smad binding site.
Dev. Biol. 244: 257-266, 2002.

5. Lazakovitch EM, She BR, Lien CL, Woo WM, Ju YT, Lin-Chao S.
The Gas7 gene encodes two protein isoforms differentially expressed within the brain. Genomics 61: 298-306, 1999.

6.Lien CL, Wu C, Mercer B, Webb R, Richardson JA, Olson EN.
Control of early cardiac-specific transcription of Nkx2-5 by a GATA-dependent enhancer.
Development 126: 75-84, 1999.

7. Cripps RM, Black BL, Zhao B., Lien CL, Schulz RA, Olson EN.
The myogenic regulatory gene Mef2 is a direct target for transcription activation by Twist during Drosophila myogenesis.
Genes & Development 12: 422-434, 1998.