PUBLICATION
Transcriptomic profiling of tissue environments critical for post-embryonic patterning and morphogenesis of zebrafish skin
- Authors
- Aman, A.J., Saunders, L.M., Carr, A.A., Srivatasan, S., Eberhard, C., Carrington, B., Watkins-Chow, D., Pavan, W.J., Trapnell, C., Parichy, D.M.
- ID
- ZDB-PUB-230912-52
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Parichy, David M.
- Keywords
- adult form, developmental biology, differentiation, morphogenesis, pigment, skin, squamation, zebrafish
- Datasets
- GEO:GSE224695
- MeSH Terms
-
- Animals
- Female
- Gene Expression Profiling
- Morphogenesis/genetics
- Skin
- Transcriptome*
- Zebrafish*/genetics
- PubMed
- 37695017 Full text @ Elife
Citation
Aman, A.J., Saunders, L.M., Carr, A.A., Srivatasan, S., Eberhard, C., Carrington, B., Watkins-Chow, D., Pavan, W.J., Trapnell, C., Parichy, D.M. (2023) Transcriptomic profiling of tissue environments critical for post-embryonic patterning and morphogenesis of zebrafish skin. eLIFE. 12:.
Abstract
Pigment patterns and skin appendages are prominent features of vertebrate skin. In zebrafish, regularly patterned pigment stripes and an array of calcified scales form simultaneously in the skin during post-embryonic development. Understanding the mechanisms that regulate stripe patterning and scale morphogenesis may lead to the discovery of fundamental mechanisms that govern the development of animal form. To learn about cell types and signaling interactions that govern skin patterning and morphogenesis, we generated and analyzed single-cell transcriptomes of skin from wild-type fish as well as fish having genetic or transgenically induced defects in squamation or pigmentation. These data reveal a previously undescribed population of epidermal cells that express transcripts encoding enamel matrix proteins, suggest hormonal control of epithelial-mesenchymal signaling, clarify the signaling network that governs scale papillae development, and identify a critical role for the hypodermis in supporting pigment cell development. Additionally, these comprehensive single-cell transcriptomic data representing skin phenotypes of biomedical relevance should provide a useful resource for accelerating the discovery of mechanisms that govern skin development and homeostasis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping