ZFIN ID: ZDB-PERS-111121-1
Chatti, Kiranam
Email: kiranamc@drils.org
URL: https://drils.org/team/kiranam-chatti/
Affiliation: Chatti Lab
Address: Dr. Reddy's Institute of Life Sciences (DRILS) University of Hyderabad Campus Gachibowli, Hyderabad - 500046 India
Country: India
Phone: +91-40-66571532
Fax: +91-40-66571581
ORCID ID: 0000-0002-1234-5271


BIOGRAPHY AND RESEARCH INTERESTS
2013 onwards
Principal Research Scientist and Zebrafish Facility Head, Center for Innovation in Molecular and Pharmaceutical Sciences, Dr. Reddy's Institute of Life Sciences, University of Hyderabad Campus, India.
2008 onwards
Senior Research Scientist at the Institute of Life Sciences, Dept. of Biology, University of Hyderabad Campus, India.
2005 - 2008
Post-Doctoral Research Associate in the State University of New York at Stony Brook, Dept. of Physiology & Biophysics, New York, USA.
1998 - 2004
Ph.D. in Pharmacology & Toxicology at the University of Mississippi Medical
Center, Jackson, Mississippi, USA.
1997 - 1998
Junior Research Fellow at the Centre for Cellular and Molecular Biology,
Hyderabad.
1995 - 1997
M.Sc. in Biotechnology at Madurai Kamaraj University, Madurai.
1992 - 1995
B.Sc. in Genetics, Botany and Chemistry at Nizam College, Osmania
University, Hyderabad, India.

Research Interests
Protein tyrosine kinases (PTKs) are cellular enzymes which catalyze phosphotransfer from ATP to tyrosine residues in proteins. The human genome encodes 90 tyrosine kinases, the majority of which are directly associated (by overexpression or mutation) with defects in cell proliferation and/or differentiation, resulting in cancer.
A current research effort in my laboratory is to understand tyrosine kinase biology in zebrafish. We have recently published the complete zebrafish tyrosine kinome highlighting their phylogenetic relationships and unique sequence features, using profile hidden markov models. We are studying the expression and role of zebrafish homologs of BRK. Understanding tyrosine kinase dysregulation and cancer in zebrafish is a likely outcome of this work.
A connected research area in my lab is based on the hypothesized regulation of tyrosine kinases by non-catalytic domain interactions with their splice-variants. We are testing this hypothesis by studying the regulation of BRK (breast tumour kinase) signalling by one of its splice-variants, and its effect on cell proliferation. The relevance of such a mechanism in cancer initiation and progression will be studied using human tissue samples.


PUBLICATIONS
Foster, S.D., Glover, S.R., Turner, A.N., Chatti, K., Challa, A.K. (2018) A mixing heteroduplex mobility assay (mHMA) to genotype homozygous mutants with small indels generated by CRISPR-Cas9 nucleases. MethodsX. 6:1-5
Vemula, M.H., Medisetti, R., Ganji, R., Jakkala, K., Sankati, S., Chatti, K., Banerjee, S. (2016) Mycobacterium tuberculosis Zinc Metalloprotease-1 Assists Mycobacterial Dissemination in Zebrafish. Frontiers in microbiology. 7:1347
Bhanushali, U., Rajendran, S., Sarma, K., Kulkarni, P., Chatti, K., Chatterjee, S., Ramaa, C.S. (2016) 5-Benzylidene-2,4-thiazolidenedione derivatives: Design, synthesis and evaluation as inhibitors of angiogenesis targeting VEGR-2. Bioorganic chemistry. 67:139-147
Kulkarni, P., Chaudhari, G.H., Sripuram, V., Banote, R.K., Kirla, K.T., Sultana, R., Rao, P., Oruganti, S., Chatti, K. (2014) Oral dosing in adult zebrafish: Proof-of-concept using pharmacokinetics and pharmacological evaluation of carbamazepine. Pharmacological reports : PR. 66:179-183
Babu, P.V., Mukherjee, S., Deora, G.S., Chennubhotla, K.S., Medisetti, R., Yellanki, S., Kulkarni, P., Sripelly, S., Parsa, K.V., Chatti, K., Mukkanti, K., and Pal, M. (2013) Ligand/PTC-free intramolecular Heck reaction: synthesis of pyrroloquinoxalines and their evaluation against PDE4/luciferase/oral cancer cell growth in vitro and zebrafish in vivo. Organic & biomolecular chemistry. 11(39):6680-5
Challa, A.K., and Chatti, K. (2013) Conservation and Early Expression of Zebrafish Tyrosine Kinases Support the Utility of Zebrafish as a Model for Tyrosine Kinase Biology. Zebrafish. 10(3):264-74
Dulla, B., Kirla, K.T., Rathore, V., Deora, G.S., Kavela, S., Maddika, S., Chatti, K., Reiser, O., Iqbal, J., and Pal, M. (2013) Synthesis and evaluation of 3-amino/guanidine substituted phenyl oxazoles as a novel class of LSD1 inhibitors with anti-proliferative properties. Organic & biomolecular chemistry. 11(19):3103-7
Banote, R.K., Koutarapu, S., Chennubhotla, K.S., Chatti, K., and Kulkarni, P. (2013) Oral gabapentin suppresses pentylenetetrazole-induced seizure-like behavior and cephalic field potential in adult zebrafish. Epilepsy & behavior : E&B. 27(1):212-219
Chaudhari, G.H., Chennubhotla, K.S., Chatti, K., and Kulkarni, P. (2013) Optimization of the adult zebrafish ECG method for assessment of drug-induced QTc prolongation. Journal of Pharmacological and Toxicological Methods. 67(2):115-120
Reddy, E.R., Banote, R.K., Chatti, K., Kulkarni, P., and Rajadurai, M.S. (2012) Selective Multicolour Imaging of Zebrafish Muscle Fibres by Using Fluorescent Organic Nanoparticles. Chembiochem : a European journal of chemical biology. 13(13):1889-1894
Rana, N., Moond, M., Marthi, A., Bapatla, S., Sarvepalli, T., Chatti, K., and Challa, A.K. (2010) Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action: an effective system for experiments in chemical biology. Zebrafish. 7(1):69-81

NON-ZEBRAFISH PUBLICATIONS
1. Kumar KS, Rambabu D, Sandra S, Kapavarapu R, Krishna GR, Basaveswara Rao MV, Chatti K, Reddy CM, Misra P, Pal M (2012). AlCl3 induced (hetero)arylation of 2,3-dichloroquinoxaline: a one-pot synthesis of mono/disubstituted quinoxalines as potential antitubercular agents.Bioorg Med Chem. 20(5):1711-22.
2. Xiang B*, Chatti K*, Qiu H, Miller WT, and Muthuswamy SK (2008). BRK is coamplified with ErbB2 to promote proliferation in breast cancer. Proc. Natl. Acad. Sci. USA 105:12463-12468. *Equal Contribution
3. Mamoon NM, Smith JK, Chatti K, Lee S, Kundrapu K, and Duhe RJ (2007). Multiple Cysteine residues are critical to Janus Kinase 2-mediated catalysis. Biochemistry 46: 14810-14818.
4. Chatti K, Farrar WL and Duhe RJ (2004). Tyrosine phosphorylation of the Janus Kinase 2 activation loop is essential for a high activity catalytic state, but dispensible for a basal catalytic state. Biochemistry 43: 4272-4283.