PUBLICATION
Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish
- Authors
- Teranikar, T., Villarreal, C., Salehin, N., Ijaseun, T., Lim, J., Dominguez, C., Nguyen, V., Cao, H., Chuong, C.J., Lee, J.
- ID
- ZDB-PUB-220831-7
- Date
- 2022
- Source
- iScience 25: 104876 (Journal)
- Registered Authors
- Cao, Hung, Lee, Juhyun
- Keywords
- Biological sciences research methodologies, Biology experimental methods, Biotechnology, Computer science
- MeSH Terms
- none
- PubMed
- 36034231 Full text @ iScience
Citation
Teranikar, T., Villarreal, C., Salehin, N., Ijaseun, T., Lim, J., Dominguez, C., Nguyen, V., Cao, H., Chuong, C.J., Lee, J. (2022) Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish. iScience. 25:104876.
Abstract
In vivo quantitative assessment of structural and functional biomarkers is essential for characterizing the pathophysiology of congenital disorders. In this regard, fixed tissue analysis has offered revolutionary insights into the underlying cellular architecture. However, histological analysis faces major drawbacks with respect to lack of spatiotemporal sampling and tissue artifacts during sample preparation. This study demonstrates the potential of light sheet fluorescence microscopy (LSFM) as a non-invasive, 4D (3days + time) optical sectioning tool for revealing cardiac mechano-transduction in zebrafish. Furthermore, we have described the utility of a scale and size-invariant feature detector, for analyzing individual morphology of fused cardiomyocyte nuclei and characterizing zebrafish ventricular contractility.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping