PUBLICATION

Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish

Authors: Teranikar, T., Villarreal, C., Salehin, N., Ijaseun, T., Lim, J., Dominguez, C., Nguyen, V., Cao, H., Chuong, C.J., Lee, J.
ID: ZDB-PUB-220831-7
Date: 2022
Source: iScience 25: 104876 (Journal)
Registered Authors: Cao, Hung, Lee, Juhyun
Keywords: Biological sciences research methodologies, Biology experimental methods, Biotechnology, Computer science
MeSH Terms: none
PubMed: 36034231 Full text @ iScience

Abstract

In vivo quantitative assessment of structural and functional biomarkers is essential for characterizing the pathophysiology of congenital disorders. In this regard, fixed tissue analysis has offered revolutionary insights into the underlying cellular architecture. However, histological analysis faces major drawbacks with respect to lack of spatiotemporal sampling and tissue artifacts during sample preparation. This study demonstrates the potential of light sheet fluorescence microscopy (LSFM) as a non-invasive, 4D (3days + time) optical sectioning tool for revealing cardiac mechano-transduction in zebrafish. Furthermore, we have described the utility of a scale and size-invariant feature detector, for analyzing individual morphology of fused cardiomyocyte nuclei and characterizing zebrafish ventricular contractility.

Genes / Markers

Figures