PUBLICATION
Hypoxia-Responsive Subtype Cells Differentiate Into Neurons in the Brain of Zebrafish Embryos Exposed to Hypoxic Stress
- Authors
- Zeng, C.W., Sheu, J.C., Tsai, H.J.
- ID
- ZDB-PUB-220302-15
- Date
- 2022
- Source
- Cell transplantation 31: 9636897221077930 (Journal)
- Registered Authors
- Tsai, Huai-Jen, Zeng, Chin-Wei
- Keywords
- brain injury, central nervous system, neural stem/progenitor cells, neurogenesis, zebrafish
- MeSH Terms
-
- Animals
- Brain
- Hypoxia/metabolism
- Neural Stem Cells*/metabolism
- Neurogenesis
- Neurons/physiology
- Zebrafish*/physiology
- PubMed
- 35225023 Full text @ Cell Transplant
Citation
Zeng, C.W., Sheu, J.C., Tsai, H.J. (2022) Hypoxia-Responsive Subtype Cells Differentiate Into Neurons in the Brain of Zebrafish Embryos Exposed to Hypoxic Stress. Cell transplantation. 31:9636897221077930.
Abstract
Severe hypoxia results in complete loss of central nervous system (CNS) function in mammals, while several other vertebrates, such as zebrafish, can regenerate after hypoxia-induced injury of CNS. Since the cellular mechanism involved in this remarkable feature of other vertebrates is still unclear, we studied the cellular regeneration of zebrafish brain, employing zebrafish embryos from transgenic line huORFZ exposed to hypoxia and then oxygen recovery. GFP-expressing cells, identified in some cells of the CNS, including some brain cells, were termed as hypoxia-responsive recovering cells (HrRCs). After hypoxia, HrRCs did not undergo apoptosis, while most non-GFP-expressing cells, including neurons, did. Major cell types of HrRCs found in the brain of zebrafish embryos induced by hypoxic stress were neural stem/progenitor cells (NSPCs) and radial glia cells (RGs), that is, subtypes of NSPCs (NSPCs-HrRCs) and RGs (RGs-HrRCs) that were induced by and sensitively responded to hypoxic stress. Interestingly, among HrRCs, subtypes of NSPCs- or RGs-HrRCs could proliferate and differentiate into early neurons during oxygen recovery, suggesting that these subtype cells might play a critical role in brain regeneration of zebrafish embryos after hypoxic stress.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping