PUBLICATION
Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte regeneration in the zebrafish heart
- Authors
- Bertozzi, A., Wu, C.C., Hans, S., Brand, M., Weidinger, G.
- ID
- ZDB-PUB-211109-22
- Date
- 2021
- Source
- Developmental Biology 481: 226-237 (Journal)
- Registered Authors
- Brand, Michael, Hans, Stefan, Weidinger, Gilbert, Wu, Chi-Chung
- Keywords
- Axin1, Cardiomyocyte, Cryoinjury, Fibrosis, Heart, Proliferation, Regeneration, Scar, Wnt signaling, Wnt/β-catenin, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation
- Myocardium/metabolism*
- Myocytes, Cardiac/metabolism*
- Regeneration*
- Wnt Signaling Pathway*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- beta Catenin/genetics
- beta Catenin/metabolism*
- PubMed
- 34748730 Full text @ Dev. Biol.
Citation
Bertozzi, A., Wu, C.C., Hans, S., Brand, M., Weidinger, G. (2021) Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte regeneration in the zebrafish heart. Developmental Biology. 481:226-237.
Abstract
Zebrafish can achieve scar-free healing of heart injuries, and robustly replace all cardiomyocytes lost to injury via dedifferentiation and proliferation of mature cardiomyocytes. Previous studies suggested that Wnt/β-catenin signaling is active in the injured zebrafish heart, where it induces fibrosis and prevents cardiomyocyte cell cycling. Here, via targeting the destruction complex of the Wnt/β-catenin pathway with pharmacological and genetic tools, we demonstrate that Wnt/β-catenin activity is required for cardiomyocyte proliferation and dedifferentiation, as well as for maturation of the scar during regeneration. Using cardiomyocyte-specific conditional inhibition of the pathway, we show that Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte proliferation. Our results stand in contrast to previous reports and rather support a model in which Wnt/β-catenin signaling plays a positive role during heart regeneration in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping