PUBLICATION

Bone morphogenetic protein signaling regulates Id1-mediated neural stem cell quiescence in the adult zebrafish brain via a phylogenetically conserved enhancer module

Authors
Zhang, G., Ferg, M., Lübke, L., Takamiya, M., Beil, T., Gourain, V., Diotel, N., Strähle, U., Rastegar, S.
ID
ZDB-PUB-200422-25
Date
2020
Source
Stem cells (Dayton, Ohio)   38(7): 875-889 (Journal)
Registered Authors
Beil, Tanja, Diotel, Nicolas, Ferg, Marco, Gourain, Victor, Lübke, Luisa, Rastegar, Sepand, Strähle, Uwe, Takamiya, Masanari, Zhang, Gaoqun
Keywords
BMP, adult neurogenesis, cis-regulatory modules, id1, neural stem cell, radial glial cell, regeneration, telencephalon, transcription, zebrafish
MeSH Terms
  • Brain/metabolism
  • Neurogenesis/genetics
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Signal Transduction
  • Neural Stem Cells*/metabolism
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism
  • Animals
(all 9)
PubMed
32246536 Full text @ Stem Cells
Abstract
In the telencephalon of adult zebrafish, the inhibitor of DNA binding 1 (id1) gene is expressed in radial glial cells (RGCs), behaving as neural stem cells (NSCs), during constitutive and regenerative neurogenesis. Id1 controls the balance between resting and proliferating states of RGCs by promoting quiescence. Here, we identified a phylogenetically conserved cis-regulatory module (CRM) mediating the specific expression of id1 in RGCs. Systematic deletion mapping and mutation of conserved transcription factor binding sites in stable transgenic zebrafish lines reveal that this CRM operates via conserved smad1/5 and 4 binding motifs under both homeostatic and regenerative conditions. Transcriptome analysis of injured and uninjured telencephala as well as pharmacological inhibition experiments identify a crucial role of bone morphogenetic protein (BMP) signaling for the function of the CRM. Our data highlight that BMP signals control id1 expression and thus NSC proliferation during constitutive and induced neurogenesis.
Genes / Markers
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Allele Construct Type Affected Genomic Region
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    Citation
    Zhang, G., Ferg, M., Lübke, L., Takamiya, M., Beil, T., Gourain, V., Diotel, N., Strähle, U., Rastegar, S. (2020) Bone morphogenetic protein signaling regulates Id1-mediated neural stem cell quiescence in the adult zebrafish brain via a phylogenetically conserved enhancer module. Stem cells (Dayton, Ohio). 38(7):875-889.