PUBLICATION

Direct activation of chordoblasts by retinoic acid is required for segmented centra mineralization during zebrafish spine development

Authors
Pogoda, H.M., Riedl-Quinkertz, I., Löhr, H., Waxman, J.S., Dale, R.M., Topczewski, J., Schulte-Merker, S., Hammerschmidt, M.
ID
ZDB-PUB-180418-31
Date
2018
Source
Development (Cambridge, England)   145(9): (Journal)
Registered Authors
Dale, Rodney M., Hammerschmidt, Matthias, Löhr, Heiko, Pogoda, Hans-Martin, Schulte-Merker, Stefan, Topczewski, Jacek, Waxman, Joshua
Keywords
Centra, Chordoblast, Notochord, Retinoic acid, Spine, Vertebral body, Zebrafish
MeSH Terms
  • Animals
  • Calcification, Physiologic/physiology*
  • Collagen/biosynthesis
  • Collagen/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Notochord/cytology
  • Notochord/embryology*
  • Retinoic Acid 4-Hydroxylase/genetics
  • Retinoic Acid 4-Hydroxylase/metabolism
  • Spine/cytology
  • Spine/embryology*
  • Tretinoin/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
29650589 Full text @ Development
Abstract
Zebrafish mutants with increased retinoic acid (RA) signaling due to the loss of the RA-inactivating enzyme Cyp26b1 develop a hyper-mineralized spine with gradually fusing vertebral body precursors (centra). However, the underlying cellular mechanisms remain incompletely understood. Here, we show that cells of the notochord epithelium named chordoblasts are sensitive to RA signaling. Chordoblasts are uniformly distributed along the anteroposterior axis and initially generate the continuous collagenous notochord sheath. However, subsequently and iteratively, subsets of these cells undergo further RA-dependent differentiation steps, acquire a stellate-like shape, downregulate expression of the collagen gene col2a1a, switch on cyp26b1 expression and trigger metameric sheath mineralization. This mineralization fails to appear upon chordoblast-specific cell ablation or RA signal transduction blockade. Together, our data reveal that, despite their different developmental origins, the activities and regulation of chordoblasts are very similar to those of osteoblasts, including their RA-induced transition from osteoid-producing cells to osteoid-mineralizing ones. Furthermore, our data point to a requirement for locally controlled RA activity within the chordoblast layer in order to generate the segmented vertebral column.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes